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RNA Scaffolds: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2323
Please select the version of book you would like to purchase.
ISBN10: 1071615017
ISBN13: 9781071615010
Publisher: Humana
Published: Jun 6 2022
Pages: 288
Weight: 1.17
Height: 0.64 Width: 7.00 Depth: 10.00
Language: English
ISBN13: 9781071615010
Publisher: Humana
Published: Jun 6 2022
Pages: 288
Weight: 1.17
Height: 0.64 Width: 7.00 Depth: 10.00
Language: English
Preface...
Table of Contents...
Contributing Authors...
Xiaojun Xu and Shi-Jie Chen 2. RNA Footprinting Using Small Chemical Reagents
Grégoire De Bisschop and Bruno Sargueil 3. Improving RNA Crystal Diffraction Quality by Post-Crystallization Treatment
Jinwei Zhang and Adrian R. Ferré-D'Amaré 4. Using tRNA Scaffold to Assist RNA Crystallization
Changrui Lu, Rujie Cai, Jason C. Grigg, and Ailong Ke 5. RNA Modeling with the Computational Energy Landscape Framework
Konstantin Röder and Samuela Pasquali 6. Co-Expression and Co-Purification of RNA-Protein Complexes in Escherichia coli
Margot El Khouri, Marjorie Catala, Bili Seijo, Johana Chabal, Frédéric Dardel, Carine Tisné, and Luc Ponchon 7. In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold
Victor G. Stepanov and George E. Fox 8. Production of Circular Recombinant RNA in Escherichia coli Using Viroid Scaffolds
José-Antonio Daròs 9. Identification of RNA-Binding Proteins Associated to RNA Structural Elements
Javier Fernandez-Chamorro, Rosario Francisco-Velilla, Azman Embarc-Buh, and Encarnacion Martinez-Salas 10. Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors
Sudeshna Manna, Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond 11. Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging
Qikun Yu, Ru Zheng, Manojkumar Narayanan, and Mingxu You 12. Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria
Cordelia A. Weiss and Wade C. Winkler 13. FRET-Analysis of RNA-Protein-Interactions using Spinach-Aptamers
Laura Roszyk and Sven Hennig 14. Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach
Charlotte Rehm, Benedikt Klauser, Monika Finke, and Jörg S. Hartig 15. Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells
Yoko Nomura and Yohei Yokobayashi 16. Folding RNA-Protein Complex into Designed Nanostructures
Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, and Hirohide Saito 17. An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA
Geunu Bak, Jee Soo Choi, Wonkyeong Kim, Shinae Suk, and Younghoon Lee 18. Expression and Purification of tRNA/pre-miRNA-Based Recombinant Noncoding RNAs
Mei-Juan Tu, Halley K. Wright, Neelu Batra, and Ai-Ming Yu 19. Synthetic Biology Medicine and Bacteria-Based Cancer Therapeutics
Jaehyung Lee, Andrew C. Keates, and Chiang J. Li Subject Index List...
Changrui Lu, Rujie Cai, Jason C. Grigg, and Ailong Ke 5. RNA Modeling with the Computational Energy Landscape Framework
Konstantin Röder and Samuela Pasquali 6. Co-Expression and Co-Purification of RNA-Protein Complexes in Escherichia coli
Margot El Khouri, Marjorie Catala, Bili Seijo, Johana Chabal, Frédéric Dardel, Carine Tisné, and Luc Ponchon 7. In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold
Victor G. Stepanov and George E. Fox 8. Production of Circular Recombinant RNA in Escherichia coli Using Viroid Scaffolds
José-Antonio Daròs 9. Identification of RNA-Binding Proteins Associated to RNA Structural Elements
Javier Fernandez-Chamorro, Rosario Francisco-Velilla, Azman Embarc-Buh, and Encarnacion Martinez-Salas 10. Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors
Sudeshna Manna, Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond 11. Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging
Qikun Yu, Ru Zheng, Manojkumar Narayanan, and Mingxu You 12. Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria
Cordelia A. Weiss and Wade C. Winkler 13. FRET-Analysis of RNA-Protein-Interactions using Spinach-Aptamers
Laura Roszyk and Sven Hennig 14. Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach
Charlotte Rehm, Benedikt Klauser, Monika Finke, and Jörg S. Hartig 15. Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells
Yoko Nomura and Yohei Yokobayashi 16. Folding RNA-Protein Complex into Designed Nanostructures
Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, and Hirohide Saito 17. An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA
Geunu Bak, Jee Soo Choi, Wonkyeong Kim, Shinae Suk, and Younghoon Lee 18. Expression and Purification of tRNA/pre-miRNA-Based Recombinant Noncoding RNAs
Mei-Juan Tu, Halley K. Wright, Neelu Batra, and Ai-Ming Yu 19. Synthetic Biology Medicine and Bacteria-Based Cancer Therapeutics
Jaehyung Lee, Andrew C. Keates, and Chiang J. Li Subject Index List...
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