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Proteomic Profiling: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2261
ISBN13: 9781071611883
Publisher: Humana
Published: Jan 10 2022
Pages: 592
Weight: 2.30
Height: 1.23 Width: 7.00 Depth: 10.00
Language: English
1. How Modern Mass Spectrometry Can Solve Ancient Questions: A multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi
Rudolf Grimm
2. Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis
Xinping Li, Thomas Franz, Ilian Atanassov, and Thomas Colby
3. Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis
Yi-Han Lin, Rodrigo Vargas Eguez, Isha Vashee, and Yanbao Yu
4. Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry
Ximo Zhang
5. Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation
Marcella Nunes Melo-Braga, MarÃa Ibáñez-Vea, Katarzyna Kulej, and Martin R. Larsen
6. Phos-Tag Fluorescent Gel Staining for the Quantitative Detection of His- and Asp-Phosphorylated Proteins
Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
7. Polyubiquitin Profile in Down Syndrome and Alzheimer Disease Brain
Antonella Tramutola and Marzia Perluigi
8. Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing
Jiaqi Wu, Will McElroy, Charles Haitjema, Carsten Lück, and Christopher Heger
9. A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes
Alin Rai, Haoyun Fang, Monique Fatmous, Bethany Claridge, Qi Hui Poh, Richard J. Simpson, and David W. Greening
10. Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma
Antonia Reale, Tiffany Khong, Rong Xu, Maoshan Chen, Sridurga Mithraprabhu, Nicholas Bingham, Andrew Spencer, and David W. Greening
11. Isolation of Extracellular Vesicles for Proteomic Profiling
Dongsic Choi, Janusz Rak, and Yong Song Gho
12. Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses
Prabal Subedi, Michael Schneider, Michael J. Atkinson, and Soile Tapio
13. Flow Cytometry as an Important Tool in Proteomic Profiling
Michael P. Blundell, Sharon L. Sanderson, and Tracey A. Long
14. Improved Immunoprecipitation to Mass Spectrometry Method for Enrichment of Low-Abundant Protein Targets
Penny Jensen, Bhavin Patel, Suzanne Smith, Renuka Sabnis, and Barbara Kaboord
15. Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors
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