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2D Page: Sample Preparation and Fractionation: Volume 1
Paperback
Series: Methods in Molecular Biology, Book 424
ISBN13: 9781617377426
Publisher: Springer Nature
Published: Dec 9 2010
Pages: 459
Weight: 1.46
Height: 0.96 Width: 6.14 Depth: 9.21
Language: English
Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. 2-DE, with its unique capacity to resolve thousands of proteins in a single run, remains a fundamental research tool for nearly all protein-related scientific projects. Step-by-step instructions alongside detailed illustrations enable biochemists, molecular and cellular biologists, and protein scientists to quickly learn how to perform the methods. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.
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