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Padrão espacial dos locais de ligação dos factores de transcrição no genoma
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ISBN10: 6207632222
ISBN13: 9786207632220
Publisher: Edicoes Nosso Conhecimento
Published: Jun 9 2024
Pages: 52
Weight: 0.20
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Portuguese
ISBN13: 9786207632220
Publisher: Edicoes Nosso Conhecimento
Published: Jun 9 2024
Pages: 52
Weight: 0.20
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Portuguese
O corpo de um mamÃfero é constituÃdo por mais de 200 tipos de células, quase todas com o mesmo genoma mas com morfologia e funções diferentes. Estas diferenças devem-se à maquinaria de regulação genética especÃfica de cada célula. O genoma dos mamÃferos tem uma organização complexa de sequências reguladoras associadas a diferentes elementos reguladores. O ADN linear no núcleo é dobrado de uma forma muito complexa e esta dobragem aproxima regiões distantes umas das outras. A combinação de diferentes proteÃnas de ligação ao ADN, como os factores de transcrição e as histonas; as marcas epigenéticas, como a posição do gene e a modificação dinâmica do ADN e das histonas; e a organização tridimensional da paisagem reguladora interagem de forma generalizada e especÃfica da célula para gerar padrões de expressão espácio-temporais complexos. As abordagens de imunoprecipitação da cromatina (ChIP seq) e de captura da conformação dos cromossomas (Hi C e 5C) podem ser integradas na sequenciação shotgun do transcriptoma completo (RNA-Seq) para decifrar o padrão espacial de ligação dos elementos reguladores aos seus genes-alvo.
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