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Análise molecular de rizobactérias diazotróficas em arroz
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ISBN10: 6630027086
ISBN13: 9786630027082
Publisher: Edicoes Nosso Conhecimento
Published: May 28 2026
Pages: 92
Weight: 0.30
Height: 0.22 Width: 6.00 Depth: 9.00
Language: Portuguese
ISBN13: 9786630027082
Publisher: Edicoes Nosso Conhecimento
Published: May 28 2026
Pages: 92
Weight: 0.30
Height: 0.22 Width: 6.00 Depth: 9.00
Language: Portuguese
Duas variedades tradicionais de arroz, Sataria e Kartki, foram colhidas em campos especÃficos localizados no distrito de Madhubani, no norte de Bihar. Uma vasta gama de bactérias fixadoras de azoto (diazotróficas) foi isolada das raÃzes destas duas variedades de arroz utilizando o enriquecimento em meios semi-sólidos sem azoto;Utilizando sondas de oligonucleótidos dirigidas ao ARN ribossómico, 53 isolados da variedade de arroz Sataria e 137 isolados da variedade de arroz Kartki foram identificados como eubactérias. Destes 190 isolados, a maioria (183) foi identificada como α-, β- ou γ- proteobactérias. Utilizando sondas de oligonucleótidos especÃficas do grupo, 14 isolados foram atribuÃdos a α-, 137 a β- e 32 a γ-subgrupo de proteobactérias. Cinco rizobactérias diazotróficas endofÃticas diferentes (Herbaspirillum seropedica, Gluconobacter diazotrophicus, Azospirillum brasilense, Burkholderia cepacia e Pseudomonas sp.) foram inoculadas em plântulas de arroz Kartki cultivadas axenicamente e o seu impacto no crescimento e na produção foi analisado. Entre as cinco bactérias diazotróficas, a B. cepacia apresentou o maior aumento no crescimento das plantas e no rendimento de grãos.
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