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Analiza molekularna ryzobakterii diazotroficznych w ryżu
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ISBN10: 6630024524
ISBN13: 9786630024524
Publisher: Wydawnictwo Nasza Wiedza
Published: May 28 2026
Pages: 88
Weight: 0.28
Height: 0.21 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786630024524
Publisher: Wydawnictwo Nasza Wiedza
Published: May 28 2026
Pages: 88
Weight: 0.28
Height: 0.21 Width: 6.00 Depth: 9.00
Language: Polish
Dwie tradycyjne odmiany ryżu, Sataria i Kartki, zostaly zebrane z określonych pól polożonych w dystrykcie Madhubani w pólnocnym Biharze. Szeroki zakres bakterii wiążących azot (diazotrofów) zostal wyizolowany z korzeni tych dwóch odmian ryżu przy użyciu wzbogacania w bezazotowe pożywki pólstale;Używając sond oligonukleotydowych ukierunkowanych na rybosomalny RNA, 53 izolaty z odmiany Sataria i 137 izolatów z odmiany ryżu Kartki zidentyfikowano jako eubakterie. Spośród tych 190 izolatów, większośc z nich (183) zidentyfikowano jako α-, β- lub γ- proteobakterie. Wykorzystując specyficzne dla grupy sondy oligonukleotydowe, 14 izolatów przypisano do α-, 137 do β- i 32 do γ-podgrupy proteobakterii. Pięc różnych endofitycznych ryzobakterii diazotroficznych (Herbaspirillum seropedica, Gluconobacter diazotrophicus, Azospirillum brasilense, Burkholderia cepacia i Pseudomonas sp.) inokulowano aksenicznie uprawianymi sadzonkami ryżu Kartki i analizowano ich wplyw na wzrost i plon. Spośród wszystkich pięciu bakterii diazotroficznych, B. cepacia wykazala najwyższy wzrost roślin i plonu ziarna.
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