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Microrna Profiling: Methods and Protocols

Microrna Profiling: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 2595

Genetics

ISBN10: 1071628224
ISBN13: 9781071628225
Publisher: Humana
Published: Nov 29 2022
Pages: 252
Weight: 1.50
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English

This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

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Genetics