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Microrna Detection and Target Identification: Methods and Protocols

Microrna Detection and Target Identification: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 2630

BiologyGenetics

ISBN10: 1071629840
ISBN13: 9781071629840
Publisher: Humana
Published: Jan 24 2024
Pages: 266
Weight: 1.07
Height: 0.58 Width: 7.00 Depth: 10.00
Language: English
This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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