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Metagenomics: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1539
BiologyGeneral ScienceGenetics
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ISBN10: 1493982745
ISBN13: 9781493982745
Publisher: Humana Pr
Published: Jul 6 2018
Pages: 303
Weight: 1.23
Height: 0.67 Width: 7.00 Depth: 10.00
Language: English
ISBN13: 9781493982745
Publisher: Humana Pr
Published: Jul 6 2018
Pages: 303
Weight: 1.23
Height: 0.67 Width: 7.00 Depth: 10.00
Language: English
Preface...
Table of Contents...
Contributing Authors...
1. Construction of Small-Insert and Large-Insert Metagenomic Libraries
Carola Simon and Rolf Daniel
2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies
Dominik Schneider, Franziska Wemheuer, Birgit Pfeiffer, and Bernd Wemheuer 3. Construction and Screening Marine Metagenomic Large Insert Libraries
Nancy Weiland-Bräuer, Daniela Langfeldt, and Ruth A. Schmitz 4. Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment
Mikkel A. Glaring, Jan K. Vester, and Peter Stougaard 5. DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms
Eleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, Özge Eyice, Hendrik Schäfer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont 6. Assessing Bacterial and Fungal Diversity in the Plant's Endosphere
Bernd Wemheuer and Franziska Wemheuer 7. Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities
James Doonan, Sandra Denman, James E. McDonald, and Peter M. Golyshin 8. Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production
Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Anné 9. Degradation Network Reconstruction Guided by Metagenomic Data
Rafael Bargiela and Manuel Ferrer 10. Novel Tools for the Functional Expression of Metagenomics DNA
Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, and Karl-Erick Jaeger 11. A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries
Dominique Böttcher, Patrick Zägel, Marlen Schmidt, and Uwe T. Bornscheuer 12. Screening for Cellulase Encoding Clones in Metagenomics Libraries
Nele Ilmberger and Wolfgang R. Streit 13. Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes
Mariette Smart, Robert J. Huddy, Don A. Cowan, and Marla Trindade 14. Screening Glycosyltransferases for Polyphenol Modifications
Nele Ilmberger and Ulrich Rabausch 15. Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial CommunitiesJiujun Cheng, Ricardo Nordeste, Maria A. Trainer, and Trevor C. Charles 16. Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity
Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel 17. Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes
Nicole Adam and Mirjam Perner 18. Screening for N-AHSL-Based-Signaling Interfering Enzymes
Stéphane Uroz and Phil M. Oger 19. Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites
F. Jerry Reen, Jose A. Gutiérrez-Barranquero, and Fergal O'Gara
Nancy Weiland-Bräuer, Daniela Langfeldt, and Ruth A. Schmitz 4. Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment
Mikkel A. Glaring, Jan K. Vester, and Peter Stougaard 5. DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms
Eleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, Özge Eyice, Hendrik Schäfer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont 6. Assessing Bacterial and Fungal Diversity in the Plant's Endosphere
Bernd Wemheuer and Franziska Wemheuer 7. Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities
James Doonan, Sandra Denman, James E. McDonald, and Peter M. Golyshin 8. Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production
Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Anné 9. Degradation Network Reconstruction Guided by Metagenomic Data
Rafael Bargiela and Manuel Ferrer 10. Novel Tools for the Functional Expression of Metagenomics DNA
Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, and Karl-Erick Jaeger 11. A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries
Dominique Böttcher, Patrick Zägel, Marlen Schmidt, and Uwe T. Bornscheuer 12. Screening for Cellulase Encoding Clones in Metagenomics Libraries
Nele Ilmberger and Wolfgang R. Streit 13. Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes
Mariette Smart, Robert J. Huddy, Don A. Cowan, and Marla Trindade 14. Screening Glycosyltransferases for Polyphenol Modifications
Nele Ilmberger and Ulrich Rabausch 15. Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial CommunitiesJiujun Cheng, Ricardo Nordeste, Maria A. Trainer, and Trevor C. Charles 16. Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity
Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel 17. Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes
Nicole Adam and Mirjam Perner 18. Screening for N-AHSL-Based-Signaling Interfering Enzymes
Stéphane Uroz and Phil M. Oger 19. Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites
F. Jerry Reen, Jose A. Gutiérrez-Barranquero, and Fergal O'Gara
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