Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
612-822-4611

  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Molecular Biology Techniques: Polymerase Chain Reaction (PCR)

Molecular Biology Techniques: Polymerase Chain Reaction (PCR)

Dantas Guimarães, Beatriz

Paperback

Genetics

ISBN10: 6208515491
ISBN13: 9786208515492
Publisher: Our Knowledge Publishing
Published: Dec 30 2024
Pages: 52
Weight: 0.20
Height: 0.12 Width: 6.00 Depth: 9.00
Language: English
The polymerase chain reaction (PCR) has as its main application the possibility of amplifying a segment of DNA, producing millions of copies of the selected segment. Gene cloning aims to introduce a selected gene into bacteria that are identical to each other, and this technique requires the presence of a cloning vector capable of amplifying a fragment of DNA cloned into them. The E gene of the dengue virus (DENV) is the most widely used for molecular studies due to the importance of the E protein. The aim of this study is to establish techniques for amplifying the E gene of DENV-2 and DENV-4. The DNA molecule was obtained from FIOCRUZ in Recife; the primers were selected in such a way as to encompass the E gene. PCR amplification was satisfactorily established for the E gene. Once determined, these techniques are essential in the stages of obtaining recombinant DNA in the process of cloning and expressing the dengue E protein.

Also from

Dantas Guimarães, Beatriz

View All

Also in

Genetics

View All