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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
HPLC Determination of Sulphacetamide Sodium & Prednisolone Acetate

HPLC Determination of Sulphacetamide Sodium & Prednisolone Acetate

Ejaz, Ejaz
Khan, Islam Ullah
Sharif, Shahzad

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Chemistry

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ISBN10: 3846502332
ISBN13: 9783846502334
Publisher: Lap Lambert Academic Pub
Published: Sep 30 2011
Pages: 60
Weight: 0.22
Height: 0.14 Width: 6.00 Depth: 9.00
Language: English
In this research work a rapid reversed phase liquid chromatographic method was developed and validated for Sulphacetamide Sodium (SCS) and Prednisolone Acetate (PAC) in ophthalmic suspension. Chromatographic separation was achieved by using ammonium acetate buffer and methanol (0.02M; pH 4.0) (30:70 V/V) at a flow rate of 1.5 ml/min. The effluent from column was monitored by means of Diode array detector at 271 nm and 245 nm for SCS & PAC. The retention time for SCS was 1.8 and for PAC was 3.6. A good linear relationship was observed over the concentration ranges 0.016-10 μg/ml and 0.64-400 μg/ml with correlation coefficient 0.9999 and 0.9999 for SCS and PAC respectively. Calculated LOD & LOQ were 15 ng/ml and 18 ng/ml and 46 ng/ml and 55 ng/ml for SCS and PAC respectively. All the excipients and degradation products were resolved in > 6 mints with R

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