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High-Throughput Next Generation Sequencing: Methods and Applications
Paperback
Series: Methods in Molecular Biology, Book 733
ISBN13: 9781493961641
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 308
Weight: 1.23
Height: 0.67 Width: 7.00 Depth: 10.00
Language: English
Part I: Genome Sequencing
1. Helicos Single Molecule Sequencing of Bacterial Genomes
Kathleen E. Steinmann, Christopher E. Hart, John F. Thompson, and Patrice M. Milos
2. Whole Genome Sequencing of Unculturable Bacterium Using Whole Genome Amplification
Yuichi Hongoh and Atsushi Toyoda
Part II: Gene Expression Analysis
3. RNA Sequencing and Quantitation Using the Helicos Genetic Analysis System
Tal Raz, Marie Causey, Daniel R. Jones, Alix Kieu, Stan Letovsky, Doron Lipson, Edward Thayer, John F. Thompson, and Patrice M. Milos
4. Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing
Fatih Ozsolak and Patrice M. Milos
5. Discovery of Bacterial sRNAs by High-Throughput Sequencing
Jane M. Liu and Andrew Camilli
6. Identification of Virus Encoding MicroRNAs Using 454 FLX Sequencing Platform
Byung-Whi Kong
7. Ribosomal RNA Depletion for Massively Parallel Bacterial RNA-Sequencing Applications
Zhoutao Chen and Xiaoping Duan
Part III: Microbial Diversity
8. Integrating High-Throughput Pyrosequencing and Quantitative Real-Time PCR to Analyze Complex Microbial Communities
Husen Zhang, Prathap Parameswaran, Jonathan Badalamenti, Bruce E. Rittmann, and Rosa Krajmalnik-Brown
9. Tag-Encoded FLX Amplicon Pyrosequencing for the Elucidation of Microbial and Functional Gene Diversity in Any Environment
Yan Sun, Randall D. Wolcott, and Scot E. Dowd
10. Pyrosequencing of Chaperonin-60 (cpn60) Amplicons as a Means of Determining Microbial Community Composition
John Schellenberg, Matthew G. Links, Janet E. Hill, Sean M. Hemmingsen, Geoffrey A. Peters, and Tim J. Dumonceaux
11. Prescreening of Microbial Populations for the Assessment of Sequencing Potential
Irene B. Hanning and Steven C. Ricke
Part IV: Metagenomics
12. Metagenomics
Jack A Gilbert, Bonnie Laverock, Ben Temperton, Simon Thomas, Martin Muhling, and Margaret Hughes
13. Metagenomic Analysis of Intestinal Microbiomes in Chickens
Taejoong Kim and Egbert Mundt
14. Gene Expression Profiling: Metatranscriptomics
Jack A. Gilbert and Margaret Hughes
Part V: Sequence Profiling for Functional Analysis
15. High-Throughput Insertion Tracking by Deep Sequencing for the Analysis of Bacterial Pathogens
Sandy M.S. Wong, Jeffrey D. Gawronski, David Lapointe, and Brian J. Akerley
16. Determining DNA Methylation Profiles Using Sequencing
Suhua Feng, Liudmilla Rubbi, Steven E. Jacobsen, and Matteo Pellegrini
Part VI: Sequencing Library Preparation
17. Preparation of Next-Generation Sequencing Libraries Using Nextera(TM) Technology: Simultaneous DNA Fragmentation and Adaptor Tagging by In Vitro Transposition
Nicholas Caruccio
18. Amplification-Free Library Preparation for Paired-End Illumina Sequencing
Iwanka Kozarewa and Daniel J. Turner
19. Target-Enrichment through Amplification of Hairpin-Ligated Universal Targets for Next-Generation Sequencing Analysis
Pallavi Singh, Rajesh Nayak, and Young Min Kwon
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