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Hdac/Hat Function Assessment and Inhibitor Development: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2589
ISBN13: 9781071627877
Publisher: Humana
Published: Oct 19 2022
Pages: 511
Weight: 2.49
Height: 1.13 Width: 7.00 Depth: 10.00
Language: English
Part I: In Vivo and 3R Models to Assess HDAC Functions and Effects of HDACi
1. Analyzing Lymphoma Development and Progression Using HDACi in Mouse Models
Eva-Maria Piskor, René Winkler, and Christian Kosan
2. Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells
3. Defined Human Leukemic CD34+ Liquid Cultures to Study HDAC/Transcriptional Repressor Complexes
Roland Windisch, Sophie Kreissig, and Christian Wichmann
4. Development of a Cellular Model Mimicking Specific HDAC Inhibitors
Lena Hess, Verena Moos, and Christian Seiser
5. Evaluation of Anti-Tumor and On-Target Activity of HDAC Inhibitors with the Zebrafish Embryo Xenograft Model
Charlotte Gatzweiler, Johannes Ridinger, Simay Ayhan, Sara Najafi, Heike Peterziel, Olaf Witt, and Ina Oehme
6. Human Platelet Lysate as Valid Cell Growth Additive to Assess Protein Acetylation
Miriam Pons, Roland Conradi, Walburgis Brenner, and Oliver H. Krämer
7. Investigating Physiopathological Roles for Sirtuins in a Mouse Model
Shimin Sun, Xiaojiao Xia, Ming Wang, and Baohua Liu
8. Patient-Derived Organoids for In Vivo Validation of In Vitro Data
Said Kdimati, Florian Bürtin, Michael Linnebacher, and Christina Susanne Mullins
Part II: Novel HDAC Inhibitors and Improved Formulations for HDAC Inhibitors
9. Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges
Marie Kühne, Susanne Hofmann, Henry Lindemann, Zoltán Cseresnyés, Andreas Dzierza, Daniel Schröder, Maren Godmann, Andreas Koschella, Christian Eggeling, Dagmar Fischer, Marc Thilo Figge, Thomas Heinze, and Thorsten Heinzel
10. Development of Pyrazine-Anilinobenzamides as Histone Deacetylase HDAC1-3 Selective Inhibitors and Biological Testing Against Pancreas Cancer Cell Lines
Mohamed Abdelsalam, Hany S. Ibrahim, Lukas Krauss, Matthes Zessin, Anita Vecchio, Sieglinde Hastreiter, Mike Schutkowski, Günter Schneider, and Wolfgang Sippl
11. Evaluation of Small Molecule HDAC Inhibitors through In Vitro and In Cellulo Approaches
Nabanita Nawar, Harsimran Kaur Garcha, Abootaleb Sedighi, Elvin D. de Araujo, and Patrick T. Gunning
12. Synthesis, Biochemical, and Cellular Evaluation of HDAC6 Targeting Proteolysis Targeting Chimeras
Salma Darwish, Tino Heimburg, Johannes Ridinger, Daniel Herp, Matthias Schmidt, Christophe Romier, Manfred Jung, Ina Oehme, and Wolfgang Sippl
13. HDACi Delivery Systems Based on Cellulose Valproate Nanoparticles
Henry Lindemann, Marie Kühne, Andreas Koschella, Maren Godmann, Thorsten Heinzel, and Thomas Heinze
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