Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Genetic Engineering of Green Fluorescent Protein for Desired Traits

Genetic Engineering of Green Fluorescent Protein for Desired Traits

Feto Naser Aliye

Paperback

Genetics

ISBN10: 3843357870
ISBN13: 9783843357876
Publisher: Lap Lambert Academic Pub
Published: Nov 29 2012
Pages: 148
Weight: 0.50
Height: 0.34 Width: 6.00 Depth: 9.00
Language: English
The book is composed of detailed principles, methods and findings of genetic engineering of colour variants of Aequorea victoria green fluorescent protein (GFP) for thermo-stability, refolding kinetics and pH-sensitivity. In addition, principles and methods to engineer the GFP to develop its destabilized version with a potential to study transient expression of cytoplasmic proteins are meticulously presented. The GFP was also used as a model substrate to elucidate the possible chaperone activity of bacterial initiation factor IF2. Of particular interest is the developed methodology, used throughout the study, to read the fluorescence of the protein in real time using RT-PCR machine while treating the sample at temperature of interest (25-99 C) for the intended period. Such method has never been developed and used before. Much more detailed, handy and innovative methodologies are addressed in the text. Thus, the book, which was forwarded by Prof. Chemeda Fininsa, is intended for graduate students, academicians, beginner and advanced researchers in the area of genetic engineering and biotechnology.

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