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Gene Synthesis: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 852
Medical ReferenceTechnology & EngineeringGenetics
ISBN13: 9781493962006
Publisher: Humana
Published: Aug 23 2016
Pages: 297
Weight: 1.20
Height: 0.65 Width: 7.00 Depth: 10.00
Language: English
Section I: Assembly of Oligonucleotides in Synthons
1. Building Block Synthesis using the Polymerase Chain Assembly (PCA) Method
Julie A. Marchand and Jean Peccoud
2. Oligonucleotide Assembly in Yeast to Produce Synthetic DNA Fragments
Daniel G. Gibson
3. TopDown Real-Time Gene Synthesis
Mo Chao Huang, Wai Chye Cheong, Hongye Ye, Mo-Huang Li
4. De novo DNA Synthesis using Single-molecule PCR
Tuval Ben Yehezkel, Gregory Linshiz, and Ehud Shapiro
Section II: Synthon Assembly
5. SLIC: A Method for Sequence and Ligation Independent Cloning
Mamie Z. Li and Stephen J. Elledge
6. Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli
Olivia Ho-Shing, Kin Lau, William Vernon, Todd T. Eckdahl, and A. Malcolm Campbell
7. Assembling DNA Fragments by USER Fusion
Narayana Annaluru, Héloïse Muller, Sivaprakash Ramalingam, Karthikeyan Kandavelou, Viktoriya London, Sarah M. Richardson, Jessica S. Dymond, Eric M. Cooper,
Joel S. Bader, Jef D. Boeke and Srinivasan Chandrasegaran
8. Fusion PCR via Novel Overlap Sequences
Kamonchai Cha-aim, Hisashi Hoshida, Tomoaki Fukunaga, and Rinji Akada
9. Using Recombineering to Generate Point Mutations: the Oligonucleotide-based Hit & Fix Method
Suhwan Chang, Stacey Stauffer and Shyam K. Sharan
10. Using Recombineering to Generate Point Mutations: galK-Based Positive-Negative Selection Method
Kajal Biswas, Stacey Stauffer and Shyam K. Sharan
11. Assembling Large DNA Segments in Yeast
Héloïse Muller, Narayana Annaluru, Joy Wu Schwerzmann, Sarah M. Richardson, Jessica S. Dymond, Eric M. Cooper, Joel S. Bader, Jef D. Boeke, Srinivasan Chandrasegaran
12. Recursive Construction of Perfect DNA Molecules and Libraries from Imperfect Oligonucleotides
Gregory Linshiz Tuval Ben Yehezkel and Ehud Shapiro
13. Cloning Whole Bacterial Genomes in Yeast
Gwynedd A. Benders
14. Production of Infectious Poliovirus from Synthetic Viral Genomes
Jeronimo Cello and Steffen Mueller
Section III: Software for gene synthesis
15. In Silico Design of Functional DNA Constructs
Alan Villalobos, Mark Welch, and Jeremy Minshull
16. Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene Synthesis
David Hoover
17. De Novo Gene Synthesis Design Using TmPrime Software
Mo-Huang Li, Marcus Bode, Mo Chao Huang, Wai Chye Cheong, Li Shi Lim
18. Design-A-Gene with GeneDesign
Sarah M. Richardson, Steffi Liu, Jef D. Boeke, and Joel S. Bader
Section IV: Education and Security
19. Leading a Successful iGEM Team
Wayne Materi
20. The Build-a-Genome Course
Eric M. Cooper, Helöise Müller, Srinivasan Chandrasegaran, Joel Bader, and Jef D. Boeke
21. DNA Synthesis Security
Ali Nouri and Christopher F. Chyba
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