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L'édition du génome avec le système CRISPR Cas9
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ISBN10: 6208348293
ISBN13: 9786208348298
Publisher: Editions Notre Savoir
Published: Dec 3 2024
Pages: 56
Weight: 0.21
Height: 0.13 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786208348298
Publisher: Editions Notre Savoir
Published: Dec 3 2024
Pages: 56
Weight: 0.21
Height: 0.13 Width: 6.00 Depth: 9.00
Language: French
L'ingénierie des génomes est à l'aube de son âge d'or. De nouveaux outils passionnants apparaissent et s'ajoutent à son arsenal. Après les TALEN et les Zing-finger, un nouvel outil digne d'intérêt vient s'ajouter. Il s'agit du système CRISPR. Ce système a été découvert pour la première fois chez le Streptococcus thermophilus et son rôle naturel est l'immunité adaptative spécifique à la séquence contre l'ADN étranger dans les bactéries. Dans le présent rapport, le système CRISPR Cas9, de type II, est utilisé pour tenter d'éliminer le gène HPRT1 exprimé dans les lymphocytes, à titre de preuve de concept. Le milieu HAT combiné à la thioguanine est utilisé comme milieu de sélection, ce qui permet une méthode de criblage rapide et simple. Pour vérifier que l'édition a bien eu lieu dans les cellules de mammifères, un kit de détection du clivage de l'ADN est utilisé en combinaison avec un test enzymatique.
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