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Du sang à l'ADN: Un récit expérimental
Paperback
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ISBN10: 6207718941
ISBN13: 9786207718948
Publisher: Editions Notre Savoir
Published: Jun 27 2024
Pages: 64
Weight: 0.23
Height: 0.15 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786207718948
Publisher: Editions Notre Savoir
Published: Jun 27 2024
Pages: 64
Weight: 0.23
Height: 0.15 Width: 6.00 Depth: 9.00
Language: French
L'extraction de l'ADN du sang est une étape essentielle pour divers processus en aval qui comprennent le dépistage parental, le séquençage de l'ADN, le clonage de gènes et d'autres méthodes de diagnostic et de recherche. L'ouvrage porte sur l'optimisation d'une méthode rentable d'extraction de l'ADN à partir d'échantillons de sang total humain. Dans cette étude, le protocole original du légendaire scientifique Friedrich Miescher a été modifié de manière à réduire considérablement les délais et les coûts. L'étude a prouvé avec succès que l'ADN du parasite du sang (Plasmodium) peut également être isolé, comme le montre la détection par PCR du parasite dans les échantillons d'ADN extraits de patients infectés. Le protocole défini dans cette étude peut être facilement adopté par n'importe quel laboratoire de biologie moléculaire et, en raison de son faible coût et des exigences techniques, des ressources limitées et du faible budget, les chercheurs peuvent facilement le reproduire en laboratoire.
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