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Essential Genes and Genomes: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2377
ISBN13: 9781071617229
Publisher: Humana
Published: Oct 29 2022
Pages: 434
Weight: 1.70
Height: 0.91 Width: 7.00 Depth: 10.00
Language: English
1. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9
Barbara Mair, Michael Aregger, Amy H.Y. Tong, Katherine S.K. Chan, and Jason Moffat
2. CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines
Kimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, and Jason R. Cantor
3. Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes
Bert Kwanten, Jasper Neggers, and Dirk Daelemans
4. Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicans
Lauren Wensing and Rebecca S. Shapiro
5. Identification of Essential Genes Using Sequential CRISPR and siRNA Screens
Luke DeHart, Oliver P. Yockey, and Jesse Bakke
6. A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines
Kohei Nishimura and Tatsuo Fukagawa
7. New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen
Xihao Liao, Xin-Hui Xing, and Chong Zhang
8. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria
Ju-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, and Cheng-Cai Zhang
9. A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human Serum
Miguel Carda-Diéguez and Carmen Amaro
10. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosa
Bradley E. Poulsen, Anne E. Clatworthy, and Deborah T. Hung
11. Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed Sequencing
Alex J. McCarthy and Peter W. Taylor
12. Using Genome Scale Mutant Libraries to Identify Essential Genes
Kevin S. Myers, Piyush Lal, Daniel R. Noguera, and Timothy J. Donohue
13. Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing
Alejandro R. Walker and Robert C. Shields
14. The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle Systems
Gayatri Sharma and Patrick D. Curtis
15. A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus
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