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    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
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Ecto-Atpases: Recent Progress on Structure and Function

Ecto-Atpases: Recent Progress on Structure and Function

Plesner, Liselotte
Kirley, Terence L.
Knowles, Aileen F.

Paperback

Medical ReferenceChemistryLawns, Trees, & Shrubs

ISBN10: 1461377293
ISBN13: 9781461377290
Publisher: Springer Nature
Published: Oct 23 2012
Pages: 294
Weight: 1.20
Height: 0.65 Width: 7.00 Depth: 10.00
Language: English
It has been known for almost 50 years that many cells carry enzymes that hydro- lyze extracellular ATP, and the term ecto-ATPase was used first by Engelhardt 40 years ago. But until the end of the 1970's, the idea of an ATPase with its ATP hydrolyzing site on the outside of the cell membrane was met with substantial skepticism since it was thought that ATP was strictly intracellular. Nevertheless, ecto-ATPase activity was dem- onstrated using a variety of intact cells. Most ecto-ATPase(s) exhibited three common 2 characteristics: 1) activation by either Ca + or Mg2+, 2) insensitivity to the commonly used inhibitors ofF-type, P-type, and V-type ATPases, and 3) ability to hydrolyze nucleo- side triphosphates and often nucleoside diphosphates as well. At the same time, the dominant ATPase activity in many plasma membrane preparations was shown to be dis- tinct from the ion-pump ATPases, but had similar enzymatic properties as the ecto-AT- Pase(s). Thus the term E-type ATPase activity has been proposed for ATPase activity exhibiting these characteristics, and it is assumed that all ecto-ATPases are E-type AT- Pases. The converse is not true, however, since soluble E-type ATPases were shown to ex- sist in plants, microorganisms, and the saliva of blood sucking insects. These enzymes could be easily purified, and exhibited very high specific activity.

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