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DNA Damage Responses: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2444
ISBN13: 9781071620656
Publisher: Humana Pr
Published: Mar 16 2023
Pages: 284
Weight: 1.15
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
This volume provides detailed methods and key approaches used to mechanistically study DNA damage, as well as the factors involved in the damage response. Chapters guide readers through proteomics and biophysical approaches, analyzing protein function, quantifying DNA replication dynamics and nucleic acid base damage, as well as biochemical reconstitution of key pathways involved in DNA repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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