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Crispr Guide RNA Design: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2162
Technology & EngineeringGenetics
ISBN13: 9781071606896
Publisher: Humana Pr
Published: Sep 16 2021
Pages: 286
Weight: 1.15
Height: 0.62 Width: 7.00 Depth: 10.00
Language: English
Part I: In Silico Design and Optimization of Guide RNA Sequences
1. Cloud-Based Design of Short Guide RNA (sgRNA) Libraries for CRISPR Experiments
Florian Heigwer and Michael Boutros
2. Web-Based CRISPR Toolkits: Cas-OFFinder, Cas-Designer, and Cas-Analyzer
Gue-Ho Hwang, Jin-Soo Kim, and Sangsu Bae
Part II: Chemically-Modified Guide RNAs
3. Chemical Modification of Guide RNAs for Improved CRISPR Activity in CD34+ Human Hematopoietic Stem and Progenitor Cells
Jenny Shapiro, Adi Tovin, Ortal Iancu, Daniel Allen, and Ayal Hendel
4. Gene Disruption Using Chemically-Modified CRISPR-Cpf1 RNA
Moira A. McMahon and Meghdad Rahdar
5. 'Split-and-Click' sgRNA
Lapatrada Taemaitree, Arun Shivalingam, Afaf H. El-Sagheer, and Tom Brown
6. Chimeric DNA-RNA Guide RNA Designs
Shuhan Lu, Ying Zhang, and Hao Yin
Part III: Expanding the CRISPR Toolbox
7. Harnessing tRNA for Processing Ability and Promoter Activity
David J.H.F. Knapp and Tudor A. Fulga
8. Targeting Noncoding RNA Domains to Genomic Loci with CRISPR-Display: Guidelines for Designing, Building, and Testing sgRNA-ncRNA Expression Constructs
David M. Shechner
9. Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs
Quentin R.V. Ferry
10. Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12a
Ha Rim Shin, Jiyeon Kweon, and Yongsub Kim
Part IV: Characterization of CRISPR Efficacy and Specificity
11. Methods for Measuring CRISPR/Cas9 DNA Cleavage in Cells
Christopher R. Cromwell, Juan Jovel, and Basil P. Hubbard
12. In Vitro Assays for Comparing the Specificity of First- and Next-Generation CRISPR/Cas9 Systems
Christopher R. Cromwell and Basil P. Hubbard
13. Profiling Genome-Wide Specificity of CRISPR-Cas9 Using Digenome-Seq
Daesik Kim and Jin-Soo Kim
14. Detection of CRISPR/Cas9-Generated Off-Target Effect by Integration-Defective Lentiviral Vector
Xiaoling Wang, Youjun Wu, and Jiing-Kuan Yee
15. Genome-Wide CRISPR Off-Target DNA Break Detection by the BLISS Method
Roberto Ballarino, Britta A.M. Bouwman, and Nicola Crosetto
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