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Clinical Biochemistry Made Easy: Volume 3: Molecular Biology and Genetics - Blueprint of Life
Paperback
Series: Clinical Biochemistry Made Easy, Book 3
Publisher: Independently Published
Published: Sep 11 2025
Pages: 310
Weight: 0.92
Height: 0.65 Width: 6.00 Depth: 9.00
Language: English
1. Introduction to Molecular Biology and Genetics
1.1 What is Molecular Biology?
1.2 Overview of the Central Dogma
1.3 Nucleic Acids: The Language of Life
1.4 Human Genome Project - Milestones and Relevance
1.5 Organization of the Human Genome (Introns, Exons, Non-coding DNA)
1.7 Story: The Code Awakens - Birth of Life's Blueprint
1.8 MCQs and SEQs on DNA-RNA basics and genome structure
2. Structure and Function of DNA & RNA
2.1 Discovery and History - Watson, Crick, Franklin
2.2 Structure of DNA: Double Helix, Base Pairing, Stability
2.3 Types of RNA: mRNA, tRNA, rRNA, miRNA, snRNA
2.4 Chromatin Structure: Nucleosomes, Histones, Epigenetic Marks
2.5 DNA Supercoiling and Packaging in Eukaryotes
2.6 Clinical Correlation: Histone Modifications in Cancer
2.7 Story: The Spiral Script - How DNA Holds the Instructions of Life
2.8 Viva Tips: One-liners for RNA types
2.9 Spotters: DNA/RNA models, histone images
3. DNA Replication, Repair, and Mutation
3.1 Semiconservative DNA Replication
3.2 Leading vs Lagging Strand - Okazaki Fragments
3.3 DNA Repair Mechanisms:
3.4 Mutations: Types (Silent, Missense, Nonsense, Frameshift, Triplet Expansion)
3.5 Mutagens and Carcinogenesis
3.6 Clinical Disorders: Xeroderma Pigmentosum, HNPCC, Ataxia-Telangiectasia
3.7 Story: The Replicator's Race - Mistakes, Mutants, and the Repair Crew
3.8 MCQs, SEQs, VIVA Cards: Mutation examples
3.9 Spot Diagnosis: Mutation types from genetic reports
4. Transcription and Translation
4.1 Transcription (DNA to RNA)
4.2 Translation (RNA to Protein)
4.3 Genetic Code: Characteristics and Clinical Application
4.4 Clinical Correlation: β-Thalassemia, Duchenne Muscular Dystrophy
4.5 Story: From Script to Symphony - The Ribosomal Orchestra
4.6 Mnemonics: Stop Codons, Codon Tables
4.7 Spotters: Transcription-translation diagrams, histological sections
4.8 OSCE: Interpreting genetic code table for amino acid sequences
5. Gene Expression and Regulation
5.1 Operon Model: Lac and Trp Operons (Prokaryotes)
5.2 Gene Expression in Eukaryotes
5.3 Epigenetic Regulation: Methylation, Acetylation
5.4 RNA Interference and miRNA
5.5 Regulation During Development and Differentiation
5.6 Clinical Relevance: Cancer Genetics, Imprinting Disorders (e.g., Prader-Willi, Angelman)
5.7 Story: The Gene Gatekeepers - When to Speak, When to Stay Silent
5.8 Viva Cards: Gene silencing and gene amplification
5.9 MCQs: Epigenetics and oncogenes
6. Recombinant DNA Technology & PCR
6.1 DNA Cloning and Vectors (Plasmid, Bacteriophage, Cosmid)
6.2 Restriction Enzymes and DNA Ligase
6.3 PCR - Principles, Steps, and Applications
6.4 Gel Electrophoresis and Southern, Northern, Western Blot
7. Clinical Genetics and Genetic Counseling
8. Molecular Diagnosis of Genetic Disorders
9. Clinical Biochemistry Applications in Genetics
Appendices & Study Aids
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