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Biolistic DNA Delivery: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 940
Medical ReferenceAnatomy & PhysiologyGenetics
ISBN13: 9781493960682
Publisher: Humana
Published: Aug 23 2016
Pages: 415
Weight: 1.64
Height: 0.88 Width: 7.00 Depth: 10.00
Language: English
Part I: Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology
1. Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun
Caixia Gao and Klaus K. Nielsen
2. Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery
Shoko Ueki, Shimpei Magori, Benoît Lacroix, and Vitaly Citovsky
3. Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues
Mulpuri Sujatha and K.B.R.S. Visarada
4. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun)
Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen
5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies
Victor Gaba, Moshe Lapidot, and Amit Gal-On
6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun
Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn
Part II: Biolistic DNA Delivery in Nematodes
7. Biolistic Transformation of Caenorhabditis elegans
Meltem Isik and Eugene Berezikov
8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans
Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher
9. Biolistic Transformation of Brugia malayi
Tarig B. Higazi and Thomas R. Unnasch
Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro
10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells
Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens
11. Biolistic Transfection of Tumor Tissue Samples
Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang
12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes
David F. Steele, Ying Dou, and David Fedida
13. Biolistic Transfection of Neurons in Organotypic Brain Slices
John A. O'Brien and Sarah C.R. Lummis
Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun
Meng-Chi Yen and Ming-Derg Lai
15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery
Ahmad Jalili
16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs
Steve Pascolo
17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter
Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz
18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice
Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus
Part V: Biolistic DNA Vaccination in Animal Disease Models
19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets
Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller
20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models
Gustaf Ahlén, Matti Sällberg, and Lars Frelin
21. Gene Gun Immunization to Combat Malaria
Elke S. Bergmann-L
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