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Unnatural Amino Acids: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 794
ISBN13: 9781493958887
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 409
Weight: 1.61
Height: 0.86 Width: 7.00 Depth: 10.00
Language: English
Part I. Synthesis of Unnatural Amino Acids
1. Preparation of Unnatural Amino Acids with Ammonia-lyases and 2,3-aminomutases
László Poppe, Csaba Paizs, Klaudia Kovács, Florin-Dan Irimie, and Beáta Vértessy
2. Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids
Paola D'Arrigo and Davide Tessaro
3. Enzymatic Production of Enantiopure Amino Acids from Mono-substituted Hydantoin Substrates
Gwynneth F. Matcher, Rosemary A. Dorrington, and Stephanie G. Burton
4. Preparation of Glutamate Analogues by Enzymatic Transamination
Thierry Gefflaut, Zeinab Assaf, and Martine Sancelme
5. Carbon-Carbon Bond Forming Enzymes for the Synthesis of Non-natural Amino Acids
Pere Clapés, Jesús Joglar, and Mariana Gutiérrez
6. Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis
Francisco Javier Las Heras-Vázquez, Josefa MarÃa Clemente-Jiménez, Sergio MartÃnez-RodrÃguez, and Felipe RodrÃguez-Vico
Part II. Application of Unnatural Amino Acids
7. NMR Analysis of Unnatural Amino Acids in Natural Antibiotics
Franca Castiglione
8. Site-specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes
Jennifer C. Peeler and Ryan A. Mehl
9. Application of Unnatural Amino Acids to the De novo Design of Selective Antibiotic Peptides
Rickey P. Hicks and Amanda L. Russell
10. Use of Unnatural Amino Acids to Probe Structure-activity Relationships and Mode-of-action of Antimicrobial Peptides
Alessandro Tossi, Marco Scocchi, Sotir Zahariev, and Renato Gennaro
Part III. Use of Unnatural Amino Acids in Protein Synthesis
11. Experimental Methods for Scanning Unnatural Amino Acid Mutagenesis
Jia Liu and Ashton T. Cropp
12. Genetic Incorporation of Unnatural Amino Acids into Proteins in Yeast
Qian Wang and Lei Wang
13. Site-specific Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells
Nobumasa Hino, Kensaku Sakamoto, and Shigeyuki Yokoyama
14. Incorporation of Unnatural Non-a-amino Acids into the N-terminus of Proteins in a Cell-free Translation System
Takahiro Hohsaka
15. Site Specific Modification of Proteins by the Staudinger-phosphite Reaction
Paul Majkut, Verena Böhrsch, Remigiusz Serwa, Michael Gerrits, and Christian P. R. Hackenberger
Part IV. D-amino Acids: Analysis and Applications
16. HPLC Methods for Determination of D-aspartate and N-methyl-D-aspartate
George H. Fisher and Mara Tsesarskaia
17. Estimation of Chronological Age from the Racemization Rate of L- and D-aspartic Acid: How to Completely Separate Enantiomers from Dentin
Toshiharu Yamamoto and Susumu Ohtani
18. Enzymatic Detection of D-amino Acids
Gianluca Molla, Luciano Piubelli, Federica Volontè, and Mirella S. Pilone
19. An Enzymatic-HPLC Assay to Monitor Endogenous D-serine Release from Neuronal Cultures
Inna Radzishevsky and Herman Wolosker
20. Electrophysiological Analysis of the Modulation of NMDA-receptors Function by d-serine and Glycine in the Central Nervous System
Fabrice Turpin, Glenn Dallérac, and Jean-Pierre Mothet
21. Biosensors for D-amino Acid Detection
Silvia Sacchi, Elena Rosini, Laura Caldinelli, and Loredano Pollegioni
22. Analysis of D-β-aspartyl Isomers at Specific Sites in Proteins
Noriko Fujii and Norihiko Fujii
23. N
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