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The P2x7 Receptor: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2510
ISBN13: 9781071623831
Publisher: Humana Pr
Published: Jul 2 2022
Pages: 394
Weight: 2.04
Height: 0.94 Width: 7.00 Depth: 10.00
Language: English
1. How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating
Steven E. Mansoor
2. Agonists, Antagonists, and Modulators of P2X7 Receptors
Christa E. Müller and Vigneshwaran Namasivayam
3. Modulation of Cell Energy Metabolism by the P2X7 Receptor
Francesco Di Virgilio, Simonetta Falzoni, Alba Clara Sarti, Paola Chiozzi, Valentina Vultaggio-Poma, and Anna Lisa Giuliani
4. Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE
Sudad Dayl and Ralf Schmid
5. Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4)
Amal Elhage, Ross J. Turner, Peter Cuthbertson, Debbie Watson, and Ronald Sluyter
6. Development of Antibody and Nanobody Tools for P2X7
Tobias Stähler, Welbeck Danquah, Melanie Demeules, Henri Gondé, Romain Hardet, Friedrich Haag, Sahil Adriouch, Friedrich Koch-Nolte, and Stephan Menzel
7. Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors
Anna Marei Mann, Waldemar Schäfer, Sahil Adriouch, Kathleen Börner, Dirk Grimm, Ingke Braren, and Friedrich Koch-Nolte
8. Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations
Tina Jooss, Birgit Kuch, Jiong Zhang, Michael Schemann, and Annette Nicke
9. Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes
Günther Schmalzing and Fritz Markwardt
10. A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors
Anna Durner and Annette Nicke
11. Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents
Xin Liang, Laura Janks, and Terrance M. Egan
12. Using Symmetrical Organic Cation Solutions to Study P2X7 Ion Permeation
Kate Dunning, Laurie Peverini, and Thomas Grutter
13. Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors
Ruth D. Murrell-Lagnado and Toshimitsu Kawate
14. Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry
Samantha Wittke, Leif Ancker, Sana Javed, and Friedrich Haag
15. Characterization of P2X7 Receptors in Human Blood Cells
Laura Hurtado-Navarro, Alberto Baroja-Mazo, and Pablo PelegrÃn
16. Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in M
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