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The Next Generation in Membrane Protein Structure Determination
Hardcover
Series: Advances in Experimental Medicine and Biology, Book 922
ISBN13: 9783319350707
Publisher: Springer
Published: Sep 5 2016
Pages: 184
Weight: 1.24
Height: 0.50 Width: 7.00 Depth: 10.00
Language: English
This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins.
- Expression screening using fluorescent proteins
- The use of detergents in membrane protein research
- The use of NMR
- Synchrotron developments in membrane protein structural biology
- Visualisation and X-ray data collection of microcrystals
- X-ray diffraction data analysis from multiple crystals
- Serial millisecond crystallography
- Serial femtosecond crystallography
- Membrane protein structures in drug discovery
The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work.
Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.
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