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Transcriptional Regulation: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 809
Medical ReferenceChemistryGenetics
ISBN13: 9781493958542
Publisher: Humana
Published: Aug 23 2016
Pages: 627
Weight: 2.42
Height: 1.30 Width: 7.00 Depth: 10.00
Language: English
Part I: Promoter Elements, Transcription Factors, and Pre-Initiation Complex (PIC) Assembly
1. Genome Wide In Vivo Crosslinking of Sequence Specific Transcription Factors
Xiao-Yong Li and Mark D. Biggin
2. Characterization of Complex Regulatory Networks and Identification of Promoter Regulatory Elements in Yeast: In Silico and Wet-Lab Approaches
Nuno P. Mira, Miguel C. Teixeira, and Isabel Sá-Correia
3. Electrophoretic Mobility Shift Assay Analysis of NFkB Transcriptional Regulation by Nuclear IkBa
Ashish Juvekar, Sitharam Ramaswami, Subrata Manna, Tzu-Pei Chang, Adeel Zubair, and Ivana Vancurova
4. Probing Endogenous RNA Polymerase II Pre-Initiation Complexes by Electrophoretic Mobility Shift Assay
Emmanuelle Wilhelm, Christopher Takacs, and Brendan Bell
5. Elucidating Protein: DNA Complex by Oligonucleotide DNA Affinity Purification
Teddy T.C. Yang and Chi-Wing Chow
6. Chromatin Immunoprecipitation (ChIP) Assay as Tool for Analyzing Transcription Factor Activity
Padmaja Gade and Dhan V. Kalvakolanu
7. Two-Step Crosslinking for Analysis of Protein-Chromatin Interactions
Bing Tian, Jun Yang, and Allan R. Brasier
8. Chromatin Immunoprecipitation Analysis of NFkB Transcriptional Regulation by Nuclear IkBa in Human Macrophages
Sitharam Ramaswami, Subrata Manna, Ashish Juvekar, Steven Kennedy, Ales Vancura, and Ivana Vancurova
9. In Vivo ChIP for the Analysis of Microdissected Tissue Samples
Chris Murgatroyd, Anke Hoffmann, and Dietmar Spengler
10. Quantification of Protein-DNA Interactions by In Vivo Chromatin Immunoprecipitation in Yeast
Amparo Pascual-Ahuir and Markus Proft
11. Mapping Protein-DNA Interactions Using ChIP-Sequencing
Charles E. Massie and Ian G. Mills
12. ChIP and ReChIP Assays: Investigating Interactions Between Regulatory Proteins, Histone Modifications, and the DNA Sequences to which They Bind
Agnieszka D. Truax and Susanna F. Greer
13. Transcriptional Regulation of Genes Via Hypoxia-Inducible Factor
Olga Roche and Michael Ohh
14. Exchange Protein Directly Activated By Cyclic AMP 1 (Epac1)-Regulated Recruitment of CCAAT/Enhancer Binding Proteins (C/EBPs) to the Suppressor of Cytokine Signalling-3 (SOCS-3) Promoter
William A. Sands, Hayley D. Woolson, Stephen J. Yarwood, and Timothy M. Palmer
Part II: Chromatin Structure
15. Computational Analysis of Promoter Elements and Chromatin Features in Yeast
John J. Wyrick
16. Chromatin Affinity Purification (ChAP)
Ryoko Harada and Alain Nepveu
17. Determination of Histone Acetylation Status by Chromatin Immunoprecipitation
Luciano Galdieri, John Moon, and Ales Vancura
18. Immunostaining of Drosophila Polytene Chromosomes to Investigate Recruitment of Chromatin Binding Proteins
Magdalena Murawska and Alexander Brehm
19. Detection of Transcriptional Activators, Co-Activators, and Chromatin Remodeling by Chromatin Immunoprecipitation Coupled with Real-Time PCR
Tamara Y. Erkina and Alexandre M. Erkine
20. Chromatin Endogenous Cleavage and Psoralen Crosslinking Assays to Analyze rRNA Gene Chromatin In Vivo
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