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Sirtuins: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1077
ISBN13: 9781493960453
Publisher: Humana
Published: Aug 23 2016
Pages: 314
Weight: 1.26
Height: 0.69 Width: 7.00 Depth: 10.00
Language: English
Part I: Methods to Generate Sirtuin Biology Tools
1. Introduction: Sirtuins in Aging and Diseases
Leonard Guarente
2. Sirtuins in Yeast: Phenotypes and Tools
Scott Tsuchiyama, Elizabeth Kwan, Weiwei Dang, Antonio Bedalov, and Brian K. Kennedy
3. Studying the C. elegans Sirtuins
Mohan Viswanathan and Heidi A. Tissenbaum
4. Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster
Jason G. Wood, Rachel Whitaker, and Stephen L. Helfand
5. Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis
Kathleen A. Hershberger, Jonathan Motley, Matthew D. Hirschey, and Kristin A. Anderson
Part II: Methods to Identify Sirtuin Substrates
6. Mass Spectrometry-Based Detection of Protein Acetylation
Yu Li, Jeffrey C. Silva, Mary E. Skinner, and David B. Lombard
7. SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome
Yue Chen, Gozde Colak, and Yingming Zhao
8. Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry
Matthew J. Rardin, Jason M. Held, and Bradford W. Gibson
9. Identification of Deacetylase Substrates with the Biotin Switch Approach
J. Will Thompson, Alex Robeson, and Joshua L. Andersen
10. Assaying Chromatin Sirtuins
Lei Zhong, Barbara Martinez-Pastor, Dafne M. Silberman, Carlos Sebastian, and Raul Mostoslavsky
Part III: Methods to Measure Sirtuin Activity
11. Measurement of Sirtuin Enzyme Activity Using a Substrate-Agnostic Fluorometric Nicotinamide Assay
Basil P. Hubbard and David A. Sinclair
12. Detecting Sirtuin-Catalyzed Deacylation Reactions Using 32P-Labeled NAD and Thin-Layer Chromatography
Anita Zhu, Xiaoyang Su, and Hening Lin
13. Chemical Acetylation And Deacetylation
Kristofer S. Fritz
14. Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD+) with High-Performance Liquid Chromatography
Jun Yoshino and Shin-ichiro Imai
15. In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity
John Dominy, Pere Puigserver, and Carles Cantó
Part IV: Methods to Study Sirtuin Biology
16. Oxygen Flux Analysis to Understand the Biological Function of Sirtuins
Dongning Wang, Michelle F. Green, Eoin McDonnell, and Matthew D. Hirschey
17. The Emerging Links Between Sirtuins and Autophagy
In Hye Lee, Jeanho Yun, and Toren Finkel
18. Methods to Study the Role of Sirtuins in Genome Stability
Paloma MartÃnez-Redondo and Alejandro Vaquero
19. Circadian Measurements of Sirtuin Biology
Kathryn Moynihan Ramsey, Alison H. Affinati, Clara B. Peek, Biliana Marcheva, Hee-Kyung Hong, and Joseph Bass
20. Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice
Jessica Curtis and Rafael de Cabo
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