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Single Molecule Enzymology: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 778
BiologyChemistryGeneral Science
ISBN13: 9781493961900
Publisher: Humana
Published: Aug 23 2016
Pages: 294
Weight: 1.21
Height: 0.66 Width: 7.00 Depth: 10.00
Language: English
1. Single Enzyme Studies: A Historical Perspective
Alex E. Knight
2. Functional Surface-Attachment in a Sandwich Geometry of GFP-Labeled Motor Proteins
Volker Bormuth, Felix Zörgibel, Erik Schäffer, and Jonathon Howard
3. Studying Kinesin's Enzymatic Cycle Using a Single-Motor Confocal Motility Assay, Employing Förster Resonance Energy Transfer
Zdenek Lansky and Erwin J.G. Peterman
4. Fluorescence Imaging with One Nanometer Accuracy: In Vitro and In Vivo Studies of Molecular Motors
Melinda Tonks Hoffman, Janet Sheung, and Paul R. Selvin
5. Snapshots of Kinesin Motors on Microtubule Tracks
Franck J. Fourniol and Carolyn A. Moores
6. Structural and Dynamic Characterization of Biochemical Processes by Atomic Force Microscopy
Frédéric Eghiaian and Iwan A.T. Schaap
7. Using Optical Tweezers to Study Fine Details of Myosin ATPase Mechanochemical Cycle
Christopher Batters and Claudia Veigel
8. Quantum Dot Labeling Strategies to Characterize Single Molecular Motors
Shane R. Nelson, M. Yusuf Ali, and David M. Warshaw
9. Imaging Individual Myosin Molecules Within Living Cells
Tatiana A. Nenasheva, Gregory I. Mashanov, Michelle Peckham, and Justin E. Molloy
10. Single Molecule Measurements Using Microneedles
Toshio Yanagida, Yoshiharu Ishii, and Akihiko Ishijima
11. Fluorescent Nucleoside Triphosphates for Single Molecule Enzymology
Christopher P. Toseland and Martin R. Webb
12. Probing the Mechanics of the Complete DNA Transcription Cycle in Real-Time Using Optical Tweezers
Christoph G. Baumann and Stephen J. Cross
13. A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases
Natalia Fili, Christopher P. Toseland, Mark S. Dillingham, Martin R. Webb, and Justin E. Molloy
14. Real-Time Single-Molecule Observation of Green Fluorescent Protein Synthesis by Immobilized Ribosomes
Ryo Iizuka, Takashi Funatsu, and Sotaro Uemura
15. Single-Molecule Measurements of Topoisomerase Activity with Magnetic Tweezers
Yeonee Seol and Keir C. Neuman
16. Assembly of Recombinant Nucleosomes on Nanofabricated DNA Curtains for Single Molecule Imaging
Ja Yil Lee and Eric C. Greene
17. Simultaneous Observation of Chemomechanical Coupling of a Molecular Motor
Takayuki Nishizaka*, Yuu Hashimoto, and Tomoko Masaike
18. Microsecond Resolution of Single Molecule Rotation Catalyzed by Molecular Motors
Tassilo Hornung, James Martin, David Spetzler, Robert Ishmukhametov, and Wayne D. Frasch
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