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Signal Transduction Protocols
Paperback
Series: Methods in Molecular Biology, Book 756
ISBN13: 9781493961085
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 430
Weight: 1.69
Height: 0.90 Width: 7.00 Depth: 10.00
Language: English
Part I. Overviews
1. Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling
Louis M. Luttrell and Terry P. Kenakin
2. Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes
Darlaine Pétrin and Terence E. Hébert
3. Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis
John K. Westwick and Jane E. Lamerdin
4. RGS-insensitive Ga Subunits: Probes of G Subtype-selective Signaling and Physiological Functions of RGS Proteins
Kuljeet Kaur, Jason M. Kehrl, Raelene A. Charbeneau, and Richard R. Neubig
5. Bioinformatic Approaches to Metabolic Pathways Analysis
Stuart Maudsley, Wayne Chadwick, Liyun Wang, Yu Zhou, Bronwen Martin, and Sung Park
Part II. Receptor - Ligand Interactions
6. Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET
Jean-Pierre Vilardaga
7. Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes
Nicolas Audet and Graciela Piñeyro
Part III. Receptor - Receptor Interactions
8. Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL)
Gisselle A. Vélez-Ruiz and Roger K. Sunahara
9. Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization
Lamia Achour, Maud Kamal, Ralf Jockers, and Stefano Marullo
10. Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization
Laëtitia Comps-Agrar, Damien Maurel, Philippe Rondard, Jean-Philippe Pin, Trinquet Eric, and Laurent Prézeau
11. Analysis of GPCR/Ion Channel Interactions
Christophe Altier and Gerald W. Zamponi
Part IV. Receptor - Effector Coupling
12. Multicolor BiFC Analysis of G Protein Complex Formation and Localization
Thomas R. Hynes, Evan A. Yost, Stacy M. Yost, and Catherine H. Berlot
13. Real-Time BRET Assays to Measure G Protein/Effector Interactions
Darlaine Pétrin, Mélanie Robitaille, and Terence E. Hébert
14. Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP
Brock F. Binkowski, Frank Fan, and Keith V. Wood
15. Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
Lianne B. Dale and Stephen S. G. Ferguson
Part V. Spatial Control of Signal Transduction
16. Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity
Charlene Depry and Jin Zhang
17. Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells
Lisa L. Gallegos and Alexandra C. Newton
18. Visualizing Receptor Endocytosis and Trafficking
Ali Salahpour and Larry S. Barak
19. Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM
Guillermo A. Yudowski and Mark von Zastrow
20. Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy
Sudha K. Shenoy
Part VI. Protein - Protein Interactions
21. Detection and Characterization of Receptor Interactions with PDZ Domains
Stefanie L. Ritter and Randy A. Hall
22. Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins
Syed M.
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