Sign-In
Cart
Sh2 Domains: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1555
Please select the version of book you would like to purchase.
ISBN10: 1493967606
ISBN13: 9781493967605
Publisher: Humana
Published: Jan 15 2017
Pages: 558
Weight: 2.65
Height: 1.25 Width: 7.00 Depth: 10.00
Language: English
ISBN13: 9781493967605
Publisher: Humana
Published: Jan 15 2017
Pages: 558
Weight: 2.65
Height: 1.25 Width: 7.00 Depth: 10.00
Language: English
Preface...
Table of Contents...
Contributing Authors...
1. Introduction: History of SH2 Domains and Their Applications
Bernard A. Liu and Kazuya Machida 2. What Have We Learned from SH2 Domains?
Bruce J. Mayer Part II Computational Approaches 3. Hidden Markov Models for Protein Domain Homology Identification and Analysis
Karl Jablonowski 4. Classification and Lineage Tracing of SH2 Domains Throughout Eukaryotes
Bernard A. Liu 5. SH2 Ligand Prediction-Guidance for In-Silico Screening
Shawn S.C. Li and Lei Li 6. An Efficient Semi-Supervised Learning Approach to Predict SH2 Domain Mediated Interactions
Kousik Kundu and Rolf Backofen 7. Proteomic Clustering Analysis of SH2 Domain DatasetsKarl Jablonowski Part III Experimental Tools 8. Expression and Production of SH2 Domain Proteins
Bernard A. Liu, Mari Ogiue-Ikeda, and Kazuya Machida 9. Expression and Purification of Soluble STAT5b/STAT3 Proteins for SH2 Domain Binding Assay
Akira Asai and Kazuyuki Takakuma 10. Purification of SOCS (Suppressors of Cytokine Signalling) SH2 Domains for Structural and Functional Studies
Nicholas P.D. Liau, Artem Laktyushin, and Jeffrey J. Babon 11. Expression and Purification of SH2 Domains Using Baculovirus Expression System
Mari Ogiue-Ikeda and Kazuya Machida 12. Functionally Altered SH2 Domains for Biochemical Studies: Loss of Function Mutant and Domain Concatenation
Mari Ogiue-Ikeda and Kazuya Machida 13. Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain
Haiming Huang, Tomonori Kaneko, Sachdev S. Sidhu, and Shawn S.C. Li Part IV Structural Analysis and Binding Assays 14. Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain
Radu Huculeci, Fabien Kieken, Abel Garcia-Pino, Lieven Buts, Nico van Nuland, and Tom Lenaerts 15. NMR Chemical Shift Mapping of SH2 Peptide Interactions
Marissa A. McKercher and Deborah S. Wuttke 16. Calorimetric Measurement of SH2 Domain Ligand Affinities
Marissa A. McKercher and Deborah S. Wuttke 17. Binding Assays Using Recombinant SH2 Domains: Far-Western, Pull Down, and Fluorescence Polarization
Kazuya Machida and Bernard A. Liu 18. In-Solution SH2 Domain Binding Assay Based on Proximity Ligation
Kazuya Machida Part V High-Throughput Screening and Proteomics 19. Alpha-Based Multiplexed Assay for Identifying SH2 Domain Antagonists
Akira Asai and Kazuyuki Takakuma 20. Characterizing SH2 Domain Specificity and Network Interactions Using SPOT Peptide Arrays
Bernard A. Liu 21. High Throughput Quantification of SH2 Domain-Phosphopeptide Interactions with Cellulose Peptide Conjugate Microarrays
Brett W. Engelmann 22. SH2 Domains as Affinity Reagents for Phosphotyrosine Protein Enrichment and Proteomic Analysis
Mi Ke, Bizhu Chu, Lin Lin, and Ruijun Tian 23. Identification of Tyrosine Phosphorylated Proteins by SH2 Domain Affinity Purification and Mass Spectrometry
Sophia Buhs, Helwe Gerull, and Peter Nollau 24. Analysis of the Global Changes in SH2 Binding Properties Using Mass Spectrometry Supported by Quantitative Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technique
Radoslaw M. Sobota 25. Using Reciprocal Protein-Peptide Array Screening to Unravel Protein Interaction Networks
Huadong Liu, Courtney Voss, and Shawn Shun-Ch
Bernard A. Liu 5. SH2 Ligand Prediction-Guidance for In-Silico Screening
Shawn S.C. Li and Lei Li 6. An Efficient Semi-Supervised Learning Approach to Predict SH2 Domain Mediated Interactions
Kousik Kundu and Rolf Backofen 7. Proteomic Clustering Analysis of SH2 Domain DatasetsKarl Jablonowski Part III Experimental Tools 8. Expression and Production of SH2 Domain Proteins
Bernard A. Liu, Mari Ogiue-Ikeda, and Kazuya Machida 9. Expression and Purification of Soluble STAT5b/STAT3 Proteins for SH2 Domain Binding Assay
Akira Asai and Kazuyuki Takakuma 10. Purification of SOCS (Suppressors of Cytokine Signalling) SH2 Domains for Structural and Functional Studies
Nicholas P.D. Liau, Artem Laktyushin, and Jeffrey J. Babon 11. Expression and Purification of SH2 Domains Using Baculovirus Expression System
Mari Ogiue-Ikeda and Kazuya Machida 12. Functionally Altered SH2 Domains for Biochemical Studies: Loss of Function Mutant and Domain Concatenation
Mari Ogiue-Ikeda and Kazuya Machida 13. Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain
Haiming Huang, Tomonori Kaneko, Sachdev S. Sidhu, and Shawn S.C. Li Part IV Structural Analysis and Binding Assays 14. Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain
Radu Huculeci, Fabien Kieken, Abel Garcia-Pino, Lieven Buts, Nico van Nuland, and Tom Lenaerts 15. NMR Chemical Shift Mapping of SH2 Peptide Interactions
Marissa A. McKercher and Deborah S. Wuttke 16. Calorimetric Measurement of SH2 Domain Ligand Affinities
Marissa A. McKercher and Deborah S. Wuttke 17. Binding Assays Using Recombinant SH2 Domains: Far-Western, Pull Down, and Fluorescence Polarization
Kazuya Machida and Bernard A. Liu 18. In-Solution SH2 Domain Binding Assay Based on Proximity Ligation
Kazuya Machida Part V High-Throughput Screening and Proteomics 19. Alpha-Based Multiplexed Assay for Identifying SH2 Domain Antagonists
Akira Asai and Kazuyuki Takakuma 20. Characterizing SH2 Domain Specificity and Network Interactions Using SPOT Peptide Arrays
Bernard A. Liu 21. High Throughput Quantification of SH2 Domain-Phosphopeptide Interactions with Cellulose Peptide Conjugate Microarrays
Brett W. Engelmann 22. SH2 Domains as Affinity Reagents for Phosphotyrosine Protein Enrichment and Proteomic Analysis
Mi Ke, Bizhu Chu, Lin Lin, and Ruijun Tian 23. Identification of Tyrosine Phosphorylated Proteins by SH2 Domain Affinity Purification and Mass Spectrometry
Sophia Buhs, Helwe Gerull, and Peter Nollau 24. Analysis of the Global Changes in SH2 Binding Properties Using Mass Spectrometry Supported by Quantitative Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technique
Radoslaw M. Sobota 25. Using Reciprocal Protein-Peptide Array Screening to Unravel Protein Interaction Networks
Huadong Liu, Courtney Voss, and Shawn Shun-Ch
Also in
Chemistry
Everything Is Tuberculosis: The History and Persistence of Our Deadliest Infection
Green, John
Hardcover
From $19.99
The Age of Alchemy: The Secret History of Chemistry from Ancient Magic to Modern Science
Chapman, Kit
Hardcover
$28.45
The Iceman Inheritance: Prehistoric Sources of Western Man's Racism, Sexism and Aggression
Bradley, Michael
Paperback
$15.15
Princeton Review AP Chemistry Premium Prep, 28th Edition: 7 Practice Tests + Digital Practice Online + Content Review
The Princeton Review
Paperback
$26.59
AP Chemistry Premium, 2027: Prep Book with 6 Practice Tests + Comprehensive Review + Online Practice
Jespersen, Neil D., PH. D.
Kerrigan, Pamela, PH. D.
Paperback
$28.49
5 Steps to a 5: AP Chemistry 2027: Practice AP Tests + AP Content Review + Digital Exams
Moore, John T.
Paperback
$27.85
Liquid Rules: The Delightful and Dangerous Substances That Flow Through Our Lives
Miodownik, Mark
Paperback
$17.09
BioGeometry Signatures: Harmonizing the Body's Subtle Energy Exchange with the Environment
Karim Dr Sc, Ibrahim
Paperback
$36.00
The Flavor Matrix: The Art and Science of Pairing Common Ingredients to Create Extraordinary Dishes
Briscione, James
Parkhurst, Brooke
Hardcover
$38.00
Herbal Pharmacy: The Science and Magic of Preparing and Administering Plant Medicine
Bancroft, Betzy
Paperback
$42.70
Stuff Matters: Exploring the Marvelous Materials That Shape Our Man-Made World
Miodownik, Mark
Paperback
$17.09
Liquid Soapmaking: Tips, Techniques and Recipes for Creating All Manner of Liquid and Soft Soap Naturally!
Thompson, Jackie
Paperback
$33.69
Periodic Tales: A Cultural History of the Elements, from Arsenic to Zinc
Aldersey-Williams, Hugh
Paperback
$18.99
The Color of North: The Molecular Language of Proteins and the Future of Life
Rizk, Shahir S.
Fink, Maggie M.
Hardcover
$26.55
The One Thing You Need to Know: The Simple Way to Understand the Most Important Ideas in Science
Chown, Marcus
Paperback
$23.74
Science and Cooking: Physics Meets Food, from Homemade to Haute Cuisine
Weitz, David
Brenner, Michael
Sörensen, Pia
Hardcover
$33.25
Sciencia: Mathematics, Physics, Chemistry, Biology, and Astronomy for All
Betts, Moff
Tweed, Matt
Watkins, Matthew
Hardcover
From $12.99
The Telomere Effect: A Revolutionary Approach to Living Younger, Healthier, Longer
Blackburn, Elizabeth
Epel, Elissa
Paperback
$20.89
Reactions: An Illustrated Exploration of Elements, Molecules, and Change in the Universe
Gray, Theodore
Paperback
$18.99
Elements: A Visual Exploration of Every Known Atom in the Universe, Book 1 of 3
Gray, Theodore
Hardcover
$33.25
Schaum's Outline of College Chemistry: 1,340 Solved Problems + 23 Videos
Krieger, Peter
Epstein, Lawrence M.
Rosenberg, Jerome
Paperback
$23.75
Reactions: An Illustrated Exploration of Elements, Molecules, and Change in the Universe, Book 3 of 3
Gray, Theodore
Hardcover
$33.25
Modern Quantum Chemistry: Introduction to Advanced Electronic Structure Theory
Szabo, Attila
Ostlund, Neil S.
Paperback
$33.25
Schaum's Outline of Organic Chemistry, Sixth Edition
Sharefkin, Jacob
Meislich, Herbert
Nechamkin, Howard
Paperback
$30.40
Physics and Chemistry Comic Book: Science Book for Kids Age 8-12 Comic Adventures & Fun Experiments
Bollino, Giulia
Paperback
$20.39
Dopamina: Cómo Una Molécula Condiciona de Quién Nos Enamoramos, Con Quién Nos Acostamos, a Quién Votamos Y ... / The Molecule of More
Lieberman, Daniel Z.
Paperback
$14.20
Exploring the World of Chemistry: From Ancient Metals to High-Speed Computers
Tiner, John Hudson
Paperback
$18.04
An Introduction to Error Analysis, Third Edition: The Study of Uncertainties in Physical Measurements
Taylor, John R.
Paperback
$118.50
Illustrated Guide to Home Chemistry Experiments: All Lab, No Lecture
Thompson, Robert Bruce
Paperback
$28.49
Rocket Girl: The Story of Mary Sherman Morgan, America's First Female Rocket Scientist
Morgan, George D.
Paperback
$18.00
Dopamina: Cómo Una Molécula Condiciona de Quién Nos Enamoramos, Con Quién Nos Acostamos, a Quién Votamos Y ... / The Molecule of More
Long, Michael E.
Lieberman, Daniel Z.
Paperback
$18.95
The Elements: An Illustrated History of the Periodic Table (100 Ponderables) Revised and Updated
Jackson, Tom
Hardcover
$23.70
Practice Makes Perfect Chemistry Review and Workbook, Second Edition
Hattori, Heather
DeWane, Marian
Paperback
$16.15
Lippincott(r) Illustrated Reviews: Biochemistry
Cline, Susan D.
Franklin, David S.
Abali, Emine Ercikan
Paperback
$128.99
The Science of Cleaning: Use the Power of Chemistry to Clean Smarter, Easier, and Safer-With Solutions for Every Kind of Dirt
Bressanini, Dario
Paperback
$23.70
Revise IB: Biology Exam Practice Workbook - Higher Level (Second Edition)
Bragg, Sarah
Jaradat, Nadia
Paperback
$36.24
Raising Elijah: Protecting Our Children in an Age of Environmental Crisis
Steingraber, Sandra
Paperback
$21.99
Chemistry: Concepts and Problems, a Self-Teaching Guide
Post, Richard
Snyder, Chad
Houk, Clifford C.
Paperback
$26.60
Schaum's Outline of General, Organic, and Biochemistry for Nursing and Allied Health
Blei, Ira
Odian, George
Paperback
$39.00
Theory and Problems for Chemistry Olympiad: Challenging Concepts in Chemistry
Nan, Zhihan
Zhang, Sheng
Paperback
$64.40
Dmt Extraction Guide: A Step-by-Step Guide to Safely Extracting DMT from Natural Sources at Home
J. Pare, Adell
Paperback
$13.19
Quantum Mechanics, Volume 1: Basic Concepts, Tools, and Applications
Cohen-Tannoudji, Claude
Diu, Bernard
Laloë, Franck
Hardcover
$275.93
Balancing Chemical Equations Worksheets (Over 200 Reactions to Balance): Chemistry Essentials Practice Workbook with Answers
McMullen, Chris
Paperback
$14.39
La Solución de Los Telómeros: Aprende a Vivir Sano Y Feliz / The Telomere Effect
Blackburn, Elizabeth
Epel, Elissa
Paperback
$20.85
Michael Faraday's The Chemical History of a Candle: With Guides to Lectures, Teaching Guides & Student Activities
DeCoste, Donald J.
Hammack, William S.
Paperback
From $9.99
The Mystery of Life's Origin: The Continuing Controversy
Bradley, Walter L.
Olsen, Roger L.
Thaxton, Charles B.
Paperback
$31.19
Molecular Biology: Principles of Genome Function
Green, Rachel R.
Greider, Carol C.
Craig, Nancy L.
Paperback
$179.99
Nature's Amazing Chemistry: Reflections on Its Complexity and Diversity
Freemantle, Michael
Hardcover
$36.95
A Text Book of Physics and Chemistry for Nurses
Bliss, Andrew Richard
Olive, Alfred Henderson
Hardcover
$42.44
Quantum Mechanics, Volume 3: Fermions, Bosons, Photons, Correlations, and Entanglement
Laloë, Franck
Cohen-Tannoudji, Claude
Diu, Bernard
Hardcover
$259.43
The Shape of Data in Chemistry: An Introduction to Chemical Graph Theory and Topological Data Analysis
Clark, Aurora
Hardcover
$135.00
Luminous Phenomena: A Story of Spontaneous Combustion, Phosphorescence and Other Cold Lights
Pentecost, Allan
Hardcover
$36.95