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Rna-Protein Complexes and Interactions: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1421
ISBN13: 9781493935895
Publisher: Humana
Published: Mar 11 2016
Pages: 288
Weight: 1.64
Height: 0.69 Width: 7.00 Depth: 10.00
Language: English
This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand. After an introductory chapter, the book continues with ways to purify RNA-protein complexes assembled in cells or in isolated cellular extracts, methods for measuring various biochemical activities of RNA-interacting proteins or ribonucleoproteins, biochemical methods for measuring direct RNA-protein contact, as well as various new or innovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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