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Ribosome Display and Related Technologies: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 805
ISBN13: 9781493958726
Publisher: Humana
Published: Aug 23 2016
Pages: 424
Weight: 1.67
Height: 0.89 Width: 7.00 Depth: 10.00
Language: English
Part I: Review
1. Ribosome Display: A Perspective
Andreas Plückthun
Part II: Translation Extract Preparation
2. Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts
James F. Zawada
Part III: Basic Ribosome Display and Related Selection Methods
3. Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate
Julie A. Douthwaite
4. Stabilized Ribosome Display for In Vitro Selection
Shuta Hara, Mingzhe Liu, Wei Wang, Muye Xu, Zha Li, and Yoshihiro Ito
5. Eukaryotic Ribosome Display with In Situ DNA Recovery
Mingyue He, Bryan M. Edwards, Damjana Kastelic, and Michael J. Taussig
6. mRNA Display Using Covalent Coupling of mRNA to Translated Proteins
Rong Wang, Steve W. Cotten, and Rihe Liu
7. SNAP Display: In Vitro Protein Evolution in Microdroplets
Miriam Kaltenbach and Florian Hollfelder
8. cDNA Display: Rapid Stabilization of mRNA Display
Shingo Ueno and Naoto Nemoto
Part IV: Applications of Ribosome Display Methods Using Natural Amino Acids
9. Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis
Leeanne Lewis and Chris Lloyd
10. Affinity Maturation of Phage Display Antibody Populations using Ribosome Display
Maria A. Groves and Adrian A. Nickson
11. Evolution of Protein Stability Using Ribosome Display
Andrew Buchanan
12. Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries
Peter Ravn
13. Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
Yuki Mochizuki and Naoto Nemoto
14. Peptide Screening Using PURE Ribosome Display
Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K. Akbar, and Takuya Ueda
15. Rapid Selection of High Affinity Binders Using Ribosome Display
Birgit Dreier and Andreas Plückthun
16. mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries
Steve W. Cotten, Jianwei Zou, Rong Wang, Bao-cheng Huang, and Rihe Liu
17. Identification of Candidate Vaccine Genes Using Ribosome Display
Liancheng Lei
18. Ribosome Display for the Selection of Sac7d Scaffolds
Barbara Mouratou, Ghislaine Béhar, Lauranne Paillard-Laurance, Stéphane Colinet, and Frédéric Pecorari
Part V: Incorporation of Non-Natural Amino Acids for Selection by Ribosome Display and Related Methods
19. Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers
Patrick C. Reid, Yuki Goto, Takayuki Katoh, and Hiroaki Suga
20. Update on Pure Translation Display with Unnatural Amino Acid Incorporation
R. Edward Watts and Anthony C. Forster
21. In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display
Zhong Ma and Matthew C.T. Hartman
Part VI: Case Studies
22. Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display
George Thom and Ralph Minter
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