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Riboregulator Design and Analysis

Riboregulator Design and Analysis

Hardcover

Series: Methods in Molecular Biology, Book 2518

BiologyChemistry

ISBN10: 1071624202
ISBN13: 9781071624203
Publisher: Humana Pr
Published: Jun 7 2022
Pages: 333
Weight: 1.81
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
This collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator systems, the book continues with sections on techniques to create switchable riboregulator systems known as riboswitches, technologies that leverage RNA-guided CRISPR-Cas systems to edit the epigenome, control gene expression, and create diagnostics, as well as computational and experimental techniques to investigate the sequence-structure-function relationship of RNA systems that can both advance fundamental understanding and rational design of riboregulators. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.

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Chappell, James

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Chemistry