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Rho Gtpases: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1821
ISBN13: 9781493986118
Publisher: Humana
Published: Jul 31 2018
Pages: 419
Weight: 2.13
Height: 0.94 Width: 7.00 Depth: 10.00
Language: English
Part I: Computational Modeling and Imaging
1. Computational Modeling of the Dynamics of Spatiotemporal Rho GTPase Signaling: A Systematic Review
Shabnam Khatibi, Karina Islas Rios, and Lan K. Nguyen
2. Uncovering Bistability in the Rac1/RhoA Signaling Network through Integrating Computational Modeling and Experimentation
Alex von Kriegsheim and Lan K. Nguyen
3. Modeling Rho GTPase Dynamics Using Boolean Logic
Joseph H.R. Hetmanski, Jean-Marc Schwartz, and Patrick T. Caswell
4. EdgeProps: A Computational Platform for Correlative Analysis of Cell Dynamics and Near-Edge Protein Activity
Anastasia Zhurikhina, Timothy Qi, Klaus M. Hahn, Timothy C. Elston, and Denis Tsygankov
5. Applying Perturbation Expectation-Maximization to Protein Trajectories of Rho GTPases
Peter K. Koo and Simon G.J. Mochrie
6. Parallel High Resolution Imaging of Leukocyte Chemotaxis under Agarose with Rho Family GTPase Biosensors
George R.R. Bell, Dean E. Natwick, and Sean R. Collins
7. Characterization of Genetically-Encoded FRET Biosensors for Rho Family GTPases
Sara K. Donnelly, Veronika Miskolci, Alice M. Garrastegui, Dianne Cox, and Louis Hodgson
8. Screening for Rho GTPase Modulators in Actin-Dependent Processes Exemplified by Phagocytosis
Daniel Schlam, Sergio Grinstein, and Spencer A. Freeman
Part II: Biochemistry and High Throughput Methods
9. An In Vitro Kinase Assay to Assess Rac1 Phosphorylation by ERK
Daniel Brandwein, Junfeng Tong, Laiji Li, Barbara Ballermann, and Zhixiang Wang
10. Methods to Study Rho GTPases Using Bacterial Toxins
Gudula Schmidt
11. Assessment of S-Glutathionylated Rac1 in Cells Using Biotin-Labeled Glutathione
Hannah Edenbaum and Jingyan Han
12. High Throughput Assay for RhoGEFs Based on the Transcreener(R) GDP Assay
Robert G. Lowery and Meera Kumar
13. Small Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases
Peter Simons, Virginie Bondu, Angela Wandinger-Ness, and Tione Buranda
Part III: Cell Types and Processes
14. Methods to Study the Roles of Rho GTPases in Platelet Function
Francisco Rivero and Simon Calaminus
15. Methods to Investigate the Role of Rho GTPases in Osteoclast Function
Anne Morel, Anne Blangy, and Virginie Vives
16. Methods to Study the Role of Cdc42, Rac1 and Rac2 in B Cell Cytoskeletal Respon
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