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Rho Gtpases: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 827
ISBN13: 9781493958962
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 407
Weight: 1.61
Height: 0.86 Width: 7.00 Depth: 10.00
Language: English
Part I. History and Phylogenesis
1. Historical Overview of Rho GTPases
Anne J. Ridley
2. Rho GTPases: Deciphering the Evolutionary History of a Complex Protein Family
Marek Eliás and VladimÃr Klimes
Part II. Biochemistry of Rho GTPases
3. Biochemical Assays to Characterize Rho GTPases
Mamta Jaiswal, Badri N. Dubey, Katja T. Koessmeier, Lothar Gremer, and Mohammad R. Ahmadian
4. How to Analyze Bacterial Toxins Targeting Rho GTPases
Heike Bielek and Gudula Schmidt
5. Assessing Ubiquitylation of Rho GTPases in Mammalian Cells
Anne Doye, Amel Mettouchi, and Emmanuel Lemichez
6. Posttranslational Lipid Modification of Rho Family Small GTPases
Natalia Mitin, Patrick J. Roberts Emily J. Chenette, and Channing J. Der
7. Analysis of the Role of RhoGDI1 and Isoprenylation in the Degradation of Rho GTPases
Etienne Boulter and Rafael Garcia-Mata
8. A Quantitative Fluorometric Approach for Measuring the Interaction of RhoGDI with Membranes and Rho GTPases
Jared Johnson, Richard A. Cerione, and Jon W. Erickson
Part III. Functional assays
9. Rho GTPases and Cancer Cell Transendothelial Migration
Nicolas Reymond, Philippe Riou, and Anne J. Ridley
10. Quantitative and Robust Assay to Measure Cell-cell Contact Assembly and Maintenance
Sébastien Nola, Jennifer C. Erasmus, and Vania M. M. Braga
11. Rho GTPase Knockout Induction in Primary Keratinocytes from Adult Mice
Esben Pedersen, Astrid Basse, Tine Lefever, Karine Peyrollier, and Cord Brakebusch
12. Rho GTPase Techniques in Osteoclastogenesis
Roland Leung and Michael Glogauer
13. Assessment of Rho GTPase Signaling During Neurite Outgrowth
Daniel Feltrin and Olivier Pertz
14. Assesment of the Role for Rho Family GTPases in NADPH Oxidase Activation
Kei Miyano and Hideki Sumimoto
Part IV. Imaging and high throughput methods
15. Multiplex Imaging of Rho GTPase Activities in Living Cells
Désirée Spiering and Louis Hodgson
16. FRET-Based Imaging of Rac and Cdc42 Activation During Fc-Receptor Mediated Phagocytosis in Macrophages
Adam D. Hoppe
17. High Throughput Flow Cytometry Bead-Based Multiplex Assay for Identification of Rho GTPase Inhibitors
Zurab Surviladze Susan M. Young, and Larry A. Sklar
18. Functional Analysis of RhoGTPase Activation and Inhibition in a Bead-Based Miniaturized Format
Michael Schmohl, Stefanie Rimmele, Peter Gierschik, Thomas O. Joos, and Nicole Schneiderhan-Marra
19. Use of Phage Display For the Identification of Molecular Sensors Specific for Activated Rho
Patrick Chinestra, Isabelle Lajoie-Mazenc, Jean-Charles Faye, and Gilles Favre
20. Identification of New Interacting Partners for Atypical Rho-GTPases: a SILAC-Based Approach
Laura Montani, Ana Filipa Domingues, and João Bettencourt Relvas
Part V. Rho GTPases in Non-Mammalian Model Organisms
21. Using Zebrafish for Studying Rho GTPases Signaling in Vivo
Shizhen Zhu and Boon Chuan Low
22. Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans
Jamie K. Alan and Erik A. Lundquist
23. Protocol for ex vivo Incubation of Drosophila Primary Post-Embryonic Haemocytes for Real-Time Analyses
Christopher J. Sampson
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