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Recombinant and in Vitro RNA Synthesis: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 941
Medical ReferenceTechnology & EngineeringChemistry
ISBN13: 9781627031127
Publisher: Humana
Published: Oct 12 2012
Pages: 274
Weight: 1.59
Height: 0.69 Width: 7.00 Depth: 10.00
Language: English
1. Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold
Luc Ponchon and Frédéric Dardel
2. Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte and Frédéric Dardel
3. Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR
Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy, and Loren D. Williams
4. General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE
Jo L. Linpinsel and Graeme L. Conn
5. Preparation of Short RNA by in vitro Transcription
Cheng Lu and Pingwei Li
6. Native RNA Purification by Gel Filtration Chromatography
Evan P. Booy, Hui Meng and Sean A. McKenna
7. Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' Ends
Johanna M. Avis, Graeme L. Conn, and Scott C. Walker
8. Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends
Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski, and Jerzy Ciesiolka
9. Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods
Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong
10. Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA
Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski and Pascale Legault
11. Affinity Purification of RNA using an ARiBo Tag
Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins, Alexis Rompré-Brodeur, Vanessa Delfosse and Pascale Legault
12. Plasmid Template Design and in vitro Transcription of Short RNAs within a 'Structure Cassette' for Structure Probing Experiments
Virginia K. Vachon and Graeme L. Conn
13. In Vitro Transcription of Modified RNAs
Stephanie L. Moon and Jeffrey Wilusz
14. End-Labeling Oligonucleotides with Chemical Tags After Synthesis
N. Ruth Zearfoss and Sean P. Ryder
15. High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA
Ya-Ming Hou
16. Se-derivatized RNAs for X-ray Crystallography
Lina Lin and Zhen Huang
17. Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs
Luigi Martino and Maria R Conte
18. Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC
Pierre Dagenais and Pascale Legault
19. Splint Ligation of RNA with T4 DNA Ligase
Christopher J. Kershaw and Raymond T. O'Keefe
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