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RNA Tagging: Methods and Protocols

RNA Tagging: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 2166

BiologyChemistry

ISBN10: 1071607146
ISBN13: 9781071607145
Publisher: Humana
Published: Jul 26 2021
Pages: 490
Weight: 1.91
Height: 1.02 Width: 7.00 Depth: 10.00
Language: English
This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Chemistry