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RNA Abundance Analysis: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2170
BiologyChemistryGeneral Science
ISBN13: 9781071607459
Publisher: Humana
Published: Aug 16 2021
Pages: 233
Weight: 0.90
Height: 0.40 Width: 8.40 Depth: 9.90
Language: English
1. CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana
Veerendra Sharma, Wenguang Zheng, Jun Huang, and David E. Cook
2. Strand-Specific RNA-Seq Applied to Malaria Samples
Xueqing Maggie Lu and Karine Le Roch
3. Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning
Marta Barcala, Carmen Fenoll, and Carolina Escobar
4. Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting
Lena Krü el, Steffen Ostendorp, Anna Ostendorp, and Julia Kehr
5. Quantitative Analysis of Plant miRNA Primary Transcripts
Jakub Dolata, Andrzej Zielezinski, Agata Stepien, Katarzyna Kruszka, Dawid Bielewicz, Andrzej Pacak, Artur Jarmolowski, Wojciech Karlowski, and Zofia Szweykowska-Kulinska
6. A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq
Dennis Chang, Lorrayne Serra, Dihong Lu, Ali Mortazavi, and Adler Dillman
7. Analysis of RBP Regulation and Co-Regulation of mRNA 3' UTR Regions in a Luciferase Reporter System
Erin L. Sternburg and Fedor V. Karginov
8. Extraction of Small RNAs by Titanium Dioxide Nanofibers
Luis A. Jimenez and Wenwan Zhong
9. Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data
Weixiong Zhang, Xuefeng Zhou, Xiang Zhou, and Jing Xia
10. Purification and Analysis of Chloroplast RNAs in Arabidopsis
Huan Wang and Hailing Jin
11. In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes
Xiaozhen Yao, Hai Huang, and Lin Xu
12. Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs
Ankur R. Bhardwaj, Ritu Pandey, Manu Agarwal, and Surekha Katiyar-Agarwal
13. Isolation of Insect Bacteriocytes as a Platform for Transcriptomic Analyses
Mélanie Ribeiro Lopes, Pierre Simonet, Gabrielle Duport, Karen Gaget, Séverine Balmand, Akiko Sugio, Jean-Christophe Simon, Nicolas Parisot, and Federica Calevro
14. Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue
Shou-Qiang Ouyang, Gyungsoon Park, Hui-Min Ji, and Katherine A. Borkovich
15. Studying RNA-Protein Interaction Using Riboproteomics
Sonali Chaturvedi and A.L.N. Rao
16. Small RNA Extraction and Quantification
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