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Quantitative Methods in Proteomics
Hardcover
Series: Methods in Molecular Biology, Book 893
ISBN13: 9781617798849
Publisher: Humana
Published: Jun 6 2012
Pages: 539
Weight: 2.60
Height: 1.30 Width: 7.20 Depth: 10.00
Language: English
Part I. Planning a Proteomic Experiment
1. Important Issues in Planning a Proteomics Experiment - Statistical Considerations of Quantitative Proteomic Data
Katharina Podwojski, Christian Stephan, and Martin Eisenacher
Part II. Quantitative Proteomics: Gel-Based Methods
2. The Whereabouts of 2D Gels in Quantitative Proteomics
Thierry Rabilloud
3. Proteome Analysis with Classical 2D-PAGE
Caroline May, Frederic Brosseron, Kathy Pfeiffer, Helmut E. Meyer, and Katrin Marcus
4. Fast and Sentitive Coomassie Staining in Quantitative Proteomics
Nadine Dyballa and Sabine Metzger
5. Silver Staining of 2D Electrophoresis Gels
Thierry Rabilloud
6. Differential Proteome Analysis using 2D-DIGE
Caroline May, Frederic Brosseron, Piotr Chartowski, Helmut E. Meyer, and Katrin Marcus
Part III. Quantitative Proteomics: MS-Based Methods
7. Quantitative Mass Spectrometry-Based Proteomics: An Overview
Miroslav Nikolov, Carla Schmidt, and Henning Urlaub
8. Robust Workflow for iTRAQ-Based Peptide and Protein Quantification
Florian Beck, Julia Maria Burkhart, Joergt Geiger, René Peiman Zahedi, and Albert Sickmann
9. Relative Protein Quantification by MS/MS Using the Tandem Mass Tag Technology
Loïc Dayon and Jean-Charles Sanchez
10. A Rapid Approach for Isobaric Peptide Termini Labeling (Rapid-IPTL)
Christian J. Koehler, Margnus . Arntzen, Achim Treumann, and Bernd Thiede
11. ICPL - Isotope Coded Protein Label
Josef Kellermann and Friedrich Lottspeich
12. Hydroponic Isotope Labeling of Entire Plants (HILEP) and High Performance Mass Spectrometry For Quantitative Plant Proteomics
Laurence V. Bindschedler, Davinia J.S. Mills, and Rainer Cramer
13. In Vivo Quantitative Proteome Profiling: Planning and Evaluation of SILAC Experiments
Marieluise Kirchner and Matthias Selbach
14. SILAC for the Study of Mammalian Cell Lines and Yeast Protein Complexes
Heike Piechura, Silke Oeljeklaus, and Bettina Warscheid
15. Post-Digestion 18O Exchange/Labeling for Quantitative Shotgun Proteomics of Membrane Proteins
Xiaoying Ye, Brian T. Luke, Donald J. Johann Jr, King C. Chan, DaRue A. Prieto, Akira Ono, Timothy D. Veenstra, and Josip Blonder
16. Application of Label Free Proteomics for Differential Analysis of Lung Carcinoma Cell Line A549
Barbara Sitek, Daniel Waldera, Gereon Poschmann, Helmut E. Meyer, and Kai Stühler
17. Absolute Quantification (AQUA) of Proteins Using Standard Peptides and Multiple Reaction Monitoring (MRM)
Carla Schmidt and Henning Urlaub
18. Absolute Multiplexed Protein Quantification Using QconCAT Technology
Philip J. Brownridge, Victoria M. Harman, Deborah M. Simpson, and Robert K. Beynon
19. A Practical Guide to the FLEXIQuant Method
Sasha Singh, Marc Kirchner, Judith Steen, and Hanno Steen
20. Label-Free Protein Quantitation Using Weighted Spectral Counting
Christine Vogel and Edward M. Marcotte
Part IV. Specific Methods - Detection and Quantification of Post-Translational Modifications
21. Discovering the Phosphoproteome of the Hydrophobic Cytochrome c Oxidase Membrane Protein Complex
Stefan Helling, Maik Hüttemann, Bernhard Kadenbach, Rabia Ramzan, Sebastian Vogt, and Katrin Marcus
22. Assay - A Quantitative Mass Spectrometry-Based Approach for Kinase Client Screening and Activity Analysis
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