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Proteomics for Biomarker Discovery: Methods and Protocols

Proteomics for Biomarker Discovery: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1959

BiologyChemistry

ISBN10: 1493991639
ISBN13: 9781493991631
Publisher: Humana
Published: Mar 10 2019
Pages: 292
Weight: 1.65
Height: 0.75 Width: 7.00 Depth: 10.00
Language: English
This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and various analytical pipelines including Data Independent Acquisition (DIA), discovery, as well as targeted and top-down proteomics analysis workflows. Bioinformatics tools and workflows to select and evaluate candidate biomarkers or combinations of biomarkers are also presented. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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