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Protein Engineering: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1685
ISBN13: 9781493973644
Publisher: Humana
Published: Oct 30 2017
Pages: 350
Weight: 1.86
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments.
The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.
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