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Protein Electrophoresis: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 869
ISBN13: 9781617798207
Publisher: Humana
Published: May 16 2012
Pages: 648
Weight: 3.00
Height: 1.60 Width: 7.30 Depth: 10.00
Language: English
1. How It All Began -- A Personal History of Gel-Electrophoresis
Oliver Smithies
2. Introduction to Protein Electrophoresis
Pothur R. Srinivas
3. Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis
J.P. Dean Goldring
4. Protein Solubilization: Attend to the Choice of Lysis Buffer
Mandy Peach, Noelle Marsh, and Daniel J. MacPhee
5. Native Polyacrylamide Gels
Claudia Arndt, Stefanie Koristka, Holger Bartsch, and Michael Bachmann
6. Cationic Electrophoresis
Engelbert Buxbaum
7. SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins
Christian Reichel
8. Tricine-SDS-PAGE
Syed R. Haider, Helen J. Reid, and Barry L. Sharp
9. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis
Marta P. Pereira Morais, John S. Fossey, Tony D. James, Jean M. H. van den Elsen
10. Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins
Marion L. Greaser and Chad M. Warren
11. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis
Henghang Tsai and Hon-chiu Eastwood Leung
12. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples
Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann
13. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies
Gyorgy Csako
14. Microchip Electrophoresis, with respect to Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease
Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy
15. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols
Mustafa Çelebier, Clara Ibáñez, Carolina Simó, and Alejandro Cifuentes
16. Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE
Yassel Ramos, Vladimir Besada, and Lila Castellanos-Serra
17. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass
Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa
18. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting
Edward P. Trieu and Ira N. Targoff
19. Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing
Yaser Dorri
20. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids
Gyorgy Csako
21. Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution
Biji T. Kurien and R. Hal Scofield
22. Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE
Hiroyuki Matsumoto, Hisao Hanju, Biji T. Kurien, and Naoka Komori
23. One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis
Bao-Shiang Lee, G.D. Lasanthi, P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta
24. Two-Dimensional Difference Gel Electrophoresis
Jonathan S. Minden
25. Pro
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