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Poly(adp-Ribose) Polymerase: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1608
ISBN13: 9781493969920
Publisher: Humana
Published: Jul 11 2017
Pages: 528
Weight: 2.56
Height: 1.19 Width: 7.00 Depth: 10.00
Language: English
Part I: Detection and Quantification of pADPr
1. Quantitation of Poly(ADP-ribose) by Isotope Dilution Mass Spectrometry
Tabea Zubel, Rita Martello, Alexander Bürkle, and Aswin Mangerich
2. Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies
Eszter M. Horvath, Zsuzsanna K. Zsengellér, and Csaba Szabo
3. Detecting and Quantifying pADPr In Vivo
Yi-Chen Lai, Rajesh K. Aneja, Margaret A. Satchell, and Robert S.B. Clark
4. Compartment-Specific Poly-ADP-Ribose Formation as a Biosensor for Subcellular NAD Pools
Magali R. VanLinden, Marc Niere, Andrey A. Nikiforov, Mathias Ziegler, and Christian Dölle
5. Cell Cycle Resolved Measurements of Poly(ADP-ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry
Jone Michelena and Matthias Altmeyer
Part II: Identification of Protein Targets
6. Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe
Rory K. Morgan and Michael S. Cohen
7. ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method
Casey M. Daniels, Shao-En Ong, and Anthony K.L. Leung
8. Using Clickable NAD+ Analogues to Label Substrate Proteins of PARPs
Lu Zhang and Hening Lin
9. Identification of Protein Substrates of Specific PARP Enzymes Using Analog-Sensitive PARP Mutants and a 'Clickable' NAD+ Analog
Bryan A. Gibson and W. Lee Kraus
10. Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography - Tandem Mass Spectrometry
Mario Leutert, Vera Bilan, Peter Gehrig, and Michael O. Hottiger
11. Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography -Tandem Mass Spectrometry
Sara C. Larsen, Mario Leutert, Vera Bilan, Rita Martello, Stephanie Jungmichel, Clifford Young, Michael O. Hottiger, and Michael L. Nielsen
Part III: Functional Analysis
12. Poly(ADP-Ribose)-Dependent Chromatin Remodeling in DNA Repair
Théo Lebeaupin, Rebecca Smith, Sébastien Huet, and Gyula Timinszky
13. Methods to Assess the Role of Poly(ADP-Ribose) Polymerases in Regulating Mitochondrial Oxidative Function
Edit Mikó, Tünde Kovács, Tamás Fodor, and Péter Bai
14. Approaches for Investigating Translational Regulation Controlled by PARP1: Biotin-Based UV-Crosslinking and Luciferase Reporter Assay
Yingbiao Ji
15. Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1)
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