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Pirna: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2509
Technology & EngineeringChemistry
ISBN13: 9781071623794
Publisher: Humana Pr
Published: Jul 8 2022
Pages: 397
Weight: 2.05
Height: 0.94 Width: 7.00 Depth: 10.00
Language: English
Part I: Methods to Study the Nature and Function of piRNAs in Non-Classical Organisms
1. Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides
Rebecca Halbach and Pascal Miesen
2. CRISPR-Mediated Genome Engineering in Aedes aegypti
Ruichen Sun, Ming Li, Conor J. McMeniman, and Omar S. Akbari
3. PIWI-Directed DNA Elimination for Tetrahymena Genetics
Salman Shehzada and Kazufumi Mochizuki
4. Planarian PIWI-piRNA Interaction Analysis Using Immunoprecipitation and piRNA-Sequencing
Makoto Kashima, Atsumi Miyata, and Norito Shibata
5. Isolation and Processing of Bovine Oocytes for Small RNA Sequencing
Minjie Tan, Helena T.A. van Tol, and Elke F. Roovers
6. 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther
Koji Koizumi and Reina Komiya
Part II: Methods to Study Roles of piRNAs in Classic Model Organisms
7. Cloning, Sequencing, and Linkage Analysis of piRNAs
Rippei Hayashi
8. Drosophila Genetic Resources for Elucidating piRNA Pathway
Kuniaki Saito
9. Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System
Chikara Takeuchi, Kensaku Murano, Mitsuru Ishikawa, Hideyuki Okano, and Yuka W. Iwasaki
Part III: Methods to Study Nuclear Regulation by Other Non-Coding RNAs
10. Whole Mount RNA FISH Combined with Immunofluorescence for Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline
Valeriya Morgunova, Maria M. Sukhova, and Alla Kalmykova
11. CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells
Akihiko Sakashita, Masaru Ariura, and Satoshi H. Namekawa
12. Method for Evaluating Effects of Non-Coding RNAs on Nucleosome Stability
Mariko Dacher, Risa Fujita, Tomoya Kujirai, and Hitoshi Kurumizaka
13. Revisiting the Glass Treatment for Single Molecule Analysis of ncRNA Function
Shuting Shen, Masahiro Naganuma, Yukihide Tomari, and Hisashi Tadakuma
14. Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification
Wan Kin Au Yeung and Hiroyuki Sasaki
15. Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seq
Abd-El Monsif Shawky, Mahmoud Dondeti, Zissimos Mourelatos, and Anastasios Vourekas
16. UPA-Seq-Based Search Method for Functional lncRNA Ca
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