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Proteomics for Biomarker Discovery

Proteomics for Biomarker Discovery

Paperback

Series: Methods in Molecular Biology, Book 1002

BiologyChemistry

ISBN10: 1493959999
ISBN13: 9781493959990
Publisher: Humana
Published: Aug 23 2016
Pages: 320
Weight: 1.29
Height: 0.70 Width: 7.00 Depth: 10.00
Language: English

With the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures. While the mass spectrometer resides at the heart of proteomics, its ability to characterize biological samples is only as good as the sample preparation and data analysis tools used in any study. In Proteomics for Biomarker Discovery, expert researchers in the field detail many of the methods which are now commonly used to study proteomics. These include methods and techniques include both label-free approaches and those that utilize stable isotopes incorporated both during cell growth or added via a chemical reaction once the proteome is extracted from the cell. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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