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Protein Microarrays: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 785
Medical ReferenceBiologyChemistry
ISBN13: 9781617792854
Publisher: Humana
Published: Aug 19 2011
Pages: 398
Weight: 2.00
Height: 1.10 Width: 7.20 Depth: 10.00
Language: English
Part I: Reverse Phase Protein Arrays
1. Reverse Phase Protein Microarrays for Clinical Applications
Mariaelena Pierobon, Claudio Belluco, and Lance A. Liotta, and Emanuel F. Petricoin III
2. Impact of Blocking and Detection Chemistries on Antibody Performance for Reverse Phase Protein Arrays
Kristi Ambroz
3. Phosphoprotein Stability in Clinical Tissue and Its Relevance for Reverse Phase Protein Microarray Technology
Virginia Espina, Claudius Mueller, and Lance A. Liotta
4. Utilization of RNAi to Validate Antibodies for Reverse Phase Protein Arrays
Heiko Mannsperger, Stefan Uhlmann, Ulrike Korf, and Özgür Sahin
5. Antibody-Mediated Signal Amplification for Reverse Phase Protein Array-Based Protein Quantification
Jan C. Brase, Heiko Mannsperger, Holger Sültmann, and Ulrike Korf
6. Reverse-Phase Protein Lysate Microarray (RPA) for the Experimental Validation of Quantitative Protein Network Models
Satoshi Nishizuka
7. Characterization of Kinase Inhibitors Using Reverse Phase Protein Arrays
Georg Martiny-Baron, Dorothea Haasen, Daniel D'Dorazio, Johannes Voshol, and Doriano Fabbro
8. Use of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues for Diagnosis and Therapy in Routine Clinical Settings
Daniela Berg, Katharina Malinowsky, Bilge Ergin, Claudia Wolff, and Karl-Friedrich Becker
9. Producing Reverse Phase Protein Microarrays from Formalin Fixed Tissues
Claudia Wolff, Claudia Schott, Katharina Malinowsky, Daniela Berg, and Karl-Friedrich Becker
10. Use of Reverse Phase Protein Microarrays to Study Protein Expression in Leukemia: Technical and Methodological Lessons Learned
Steven M. Kornblau and Kevin C. Coombes
Part II: Antibody Microarrays
11. Antibody Microarrays as Tools for Biomarker Discovery
Marta Sanchez-Carbayo
12. Assessment of Antibody Specificity Using Suspension Bead Arrays
Jochen M. Schwenk and Peter Nilsson
13. Quantitative Analysis of Phosphoproteins Using Microspot Immunoassays
Frauke Henjes, Frank Götschel, Anika Jöcker, and Ulrike Korf
14. Robust Protein Profiling with Complex Antibody Microarrays in a Dual-Color Mode
Christoph Schröder, Mohamed S.S. Alhamdani, Kurt Fellenberg, Andrea Bauer, Anette Jacob, and Jörg D. Hoheisel
15. High-Throughput Studies of Protein Glycoforms Using Antibody-Lectin Sandwich Arrays
Brian B. Haab and Tingting Yue
16. Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies
Johanna Sonntag, Heiko Mannsperger, Anika Joecker, and Ulrike Korf
17. Recombinant Antibodies for the Generation of Antibody Arrays
Carl A.K. Borrebaeck and Christer Wingren
Part III: Protein Microarrays
18. Producing Protein Microarrays from DNA Microarrays
Oda Stoevesandt, Michael J. Taussig, and Mingyue He
19. Cell Arrays and High-Content Screening
Holger Erfle, Anastasia Eskova, Jürgen Reymann, and Vytaute Starkuviene
20. Probing Calmodulin Protein-Protein Interactions Using High Content Protein Arrays
David J. O'Connell, Mikael Bauer, Sara Linse, and Dolores J. Cahill
21. Protein Function Mi
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