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Protein Expression in Mammalian Cells: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 801
ISBN13: 9781493961603
Publisher: Humana
Published: Aug 23 2016
Pages: 276
Weight: 1.12
Height: 0.61 Width: 7.00 Depth: 10.00
Language: English
1. Why Proteins in Mammalian Cells?
James L. Hartley
2. Large Scale Transfection of Mammalian Cells
Lucia Baldi, David L. Hacker, Carine Meerschman, and Florian M. Wurm
3. Selection of High Expressing Mammalian Cells by Surface Display of Reporters
Christine T. DeMaria
4. Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles
Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash
5. Transfection of Difficult-to-Transfect Primary Mammalian Cells
Oliver Gresch and Ludger Altrogge
6. Stable Protein Expression in Mammalian Cells Using Baculoviruses
Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch
7. Using Matrix Attachment Regions to Improve Recombinant Protein Production
Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, Pierre-Alain Girod, and Nicolas Mermod
8. Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells
Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, and Tina M. Sauerwald
9. Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells
Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang
10. Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells
Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, Rebecca Hamer, and Raymond J. Owens
11. Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker
Say Kong Ng
12. Tools for Co-Producing Multiple Proteins in Mammalian Cells
Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia
13. Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases
Paula Magnelli, Alicia Bielik, and Ellen Guthrie
14. Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA
Renate Kunert and Karola Vorauer-Uhl
15. Methods for Constructing Clones for Protein Expression in Mammalian Cells
Takefumi Sone and Fumio Imamoto
16. Optimizing Transient Recombinant Protein Expression in Mammalian Cells
Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito
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