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PCR Technology: Current Innovations, Third Edition
Paperback
Medical ReferenceBiologyGeneral Law
ISBN13: 9781138198586
Publisher: CRC Press
Published: Oct 26 2016
Pages: 472
Weight: 1.78
Height: 0.95 Width: 7.00 Depth: 10.00
Language: English
A huge amount of innovation surrounds PCR as a molecular technique, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. This volume provides a critical reference point for anyone wishing to use this technology. Topics include the purification and handling of PCR templates, the effect of the manufacture and purification of the oligonucleotide on PCR behavior, optimum buffer composition, probe options, and the design and optimization of qPCR assays. The book also explores the development and refinement of instrumentation and effective controls to protect against uncertainties due to reaction variability.
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