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Organelle Proteomics
Paperback
Series: Methods in Molecular Biology, Book 432
ISBN13: 9781617377723
Publisher: Springer Nature
Published: Nov 19 2010
Pages: 421
Weight: 1.35
Height: 0.89 Width: 6.14 Depth: 9.21
Language: English
Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. This is the first book that examines organelle proteomics in depth. Detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Also, devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, the book allows scientists to gain a vital and important understanding of organelle study.
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